TY - JOUR
T1 - Comprehensive Analysis of Acylcarnitine Species in db/db Mouse Using a Novel Method of High-Resolution Parallel Reaction Monitoring Reveals Widespread Metabolic Dysfunction Induced by Diabetes
AU - Xiang, Li
AU - Wei, Juntong
AU - Tian, Xiao Yu
AU - Wang, Bei
AU - Chan, Wan
AU - Li, Shangfu
AU - Tang, Zhi
AU - Zhang, Hongsong
AU - Cheang, Wai San
AU - Zhao, Qian
AU - Zhao, Hongzhi
AU - Yang, Zhiyi
AU - Hong, Yanjun
AU - Huang, Yu
AU - Cai, Zongwei
N1 - Funding Information:
The authors would like to acknowledge the financial support from the RGC-CRF (CUHK2/CRF/12G and C2014-14EF) and IRMC/13-14/03 from Research Committee of Hong Kong Baptist University.
Publisher copyright:
© 2017 American Chemical Society
PY - 2017/10/3
Y1 - 2017/10/3
N2 - Acylcarnitines are exerting a variety of biological functions depending on the differences in lengths, saturation levels, and conjugation groups, which to a great extent contribute to the challenges of acylcarnitines quantifications due to various kinds of isomers. Here, we describe a novel method by using high-resolution parallel reaction monitoring (PRM) liquid chromatography-tandem mass spectrometry (LC-MS/MS). Both reversed-phase and normal-phase column were used in order to get accurate, reliable, widespread quantification of acylcarnitines, and without tedious sample preparation procedure. The method provided the most comprehensive acylcarnitine profile with high-resolution MS and MS/MS confirmation to date. A total of 117 acylcarnitines were detected from plasma and urine samples. The application of targeted profiling of acylcarnitines in db/m+ control and db/db diabetic mice indicated incomplete amino acid and fatty acid oxidation on diabetic mice. Interestingly, the reduction of medium odd-numbered chain acylcarnitines in urine samples was first observed between db/m+ and db/db mice. The high-resolution PRM method makes it possible to monitor the widespread metabolic changes of the acylcarnitines in response to stimuli. Besides, the accurate MS and MS/MS spectra data of the 117 acylcarnitines could be used as mass spectrometric resources for the identification of acylcarnitines.
AB - Acylcarnitines are exerting a variety of biological functions depending on the differences in lengths, saturation levels, and conjugation groups, which to a great extent contribute to the challenges of acylcarnitines quantifications due to various kinds of isomers. Here, we describe a novel method by using high-resolution parallel reaction monitoring (PRM) liquid chromatography-tandem mass spectrometry (LC-MS/MS). Both reversed-phase and normal-phase column were used in order to get accurate, reliable, widespread quantification of acylcarnitines, and without tedious sample preparation procedure. The method provided the most comprehensive acylcarnitine profile with high-resolution MS and MS/MS confirmation to date. A total of 117 acylcarnitines were detected from plasma and urine samples. The application of targeted profiling of acylcarnitines in db/m+ control and db/db diabetic mice indicated incomplete amino acid and fatty acid oxidation on diabetic mice. Interestingly, the reduction of medium odd-numbered chain acylcarnitines in urine samples was first observed between db/m+ and db/db mice. The high-resolution PRM method makes it possible to monitor the widespread metabolic changes of the acylcarnitines in response to stimuli. Besides, the accurate MS and MS/MS spectra data of the 117 acylcarnitines could be used as mass spectrometric resources for the identification of acylcarnitines.
UR - http://www.scopus.com/inward/record.url?scp=85030694878&partnerID=8YFLogxK
U2 - 10.1021/acs.analchem.7b02283
DO - 10.1021/acs.analchem.7b02283
M3 - Journal article
C2 - 28859482
AN - SCOPUS:85030694878
SN - 0003-2700
VL - 89
SP - 10368
EP - 10375
JO - Analytical Chemistry
JF - Analytical Chemistry
IS - 19
ER -
