TY - JOUR
T1 - Complex effects of two presumably antagonistic endocrine disrupting compounds on the goldfish Carassius aumtus
T2 - A comprehensive study with multiple toxicological endpoints
AU - Wu, Fengxia
AU - Lin, Li
AU - Qiu, Jian Wen
AU - Chen, Hao
AU - Weng, Shaoping
AU - Luan, Tiangang
N1 - Funding Information:
This work was supported by Special Research Fund for the Doctoral Program of Higher Education (No. 20090171110041), National Science Foundation of China (NSFC No. 21277177 ), Research Funds of Zhuhai Science and Technology Project (No. 2010B040102043 ) and Administration of Ocean and Fisheries of Guangdong Province . We sincerely thank our lab members. We also would like to gratefully acknowledge technical support from the Microscopy and Imaging Center, City University of Hong Kong .
PY - 2014/10
Y1 - 2014/10
N2 - We studied the effects of endocrine disrupting compounds nonylphenol (NP) and letrozole (LE) on the male goldfish Carassius aumtus. Exposure to NP (20μgl-1) alone caused a significant up-regulation in the expression of aromatase, estrogen receptors and vitellogenin (VTG) genes, an increase in hepatic and plasma VTG concentration, but no obvious testicular impairment. Exposure to LE (1mgkg-1) alone resulted in a significant decline in aromatase activity, reduced levels of plasma 17β-estradiol (E2), and enhanced sperm maturation. Co-exposure with LE (1mgkg-1) could only partially affect some of the estrogenic effects caused by NP (20μgl-1) (i.e. expression of hepatic and brain estrogen receptor genes, hepatic VTG concentration), but inhibit other estrogenic effects (i.e. brain and testicular aromatase activity, plasma E2). In addition, co-exposure resulted in impairment of liver mitochondria (i.e. detachment of ridges from the membrane, and uneven distribution of the cytoplasm with clusters of glycogen granules), but did not cause significant damage to the testes (i.e. the morphology, the spermatogonia and spermatozoa densities). Our results clearly showed that nonylphenol and letrozole co-exposure could induce profound effects on fish, and highlighted the importance of adopting multiple toxicological endpoints when evaluating the combined effects of endocrine disrupting compounds.
AB - We studied the effects of endocrine disrupting compounds nonylphenol (NP) and letrozole (LE) on the male goldfish Carassius aumtus. Exposure to NP (20μgl-1) alone caused a significant up-regulation in the expression of aromatase, estrogen receptors and vitellogenin (VTG) genes, an increase in hepatic and plasma VTG concentration, but no obvious testicular impairment. Exposure to LE (1mgkg-1) alone resulted in a significant decline in aromatase activity, reduced levels of plasma 17β-estradiol (E2), and enhanced sperm maturation. Co-exposure with LE (1mgkg-1) could only partially affect some of the estrogenic effects caused by NP (20μgl-1) (i.e. expression of hepatic and brain estrogen receptor genes, hepatic VTG concentration), but inhibit other estrogenic effects (i.e. brain and testicular aromatase activity, plasma E2). In addition, co-exposure resulted in impairment of liver mitochondria (i.e. detachment of ridges from the membrane, and uneven distribution of the cytoplasm with clusters of glycogen granules), but did not cause significant damage to the testes (i.e. the morphology, the spermatogonia and spermatozoa densities). Our results clearly showed that nonylphenol and letrozole co-exposure could induce profound effects on fish, and highlighted the importance of adopting multiple toxicological endpoints when evaluating the combined effects of endocrine disrupting compounds.
KW - Biomarker
KW - Endocrine disruption
KW - Environmental health
KW - Letrozole
KW - Nonylphenol
KW - Teleost
UR - http://www.scopus.com/inward/record.url?scp=84903530647&partnerID=8YFLogxK
U2 - 10.1016/j.aquatox.2014.06.003
DO - 10.1016/j.aquatox.2014.06.003
M3 - Journal article
C2 - 24974122
AN - SCOPUS:84903530647
SN - 0166-445X
VL - 155
SP - 43
EP - 51
JO - Aquatic Toxicology
JF - Aquatic Toxicology
ER -
