TY - JOUR
T1 - Comparison of the chemical profiles and anti-platelet aggregation effects of two “Dragon's Blood” drugs used in traditional Chinese medicine
AU - Yi, Tao
AU - Chen, Hu-Biao
AU - Zhao, Zhong-Zhen
AU - Yu, Zhi-Ling
AU - Jiang, Zhi-Hong
N1 - Funding Information:
This research was funded by the Faculty Research Grant of Hong Kong Baptist University ( FRG/08-09/II-52 ).
PY - 2011/1/27
Y1 - 2011/1/27
N2 - Ethnopharmacological relevance: "Dragon's Blood" has been used as a medicine since ancient times by many cultures. In traditional Chinese medicine, the resin obtained from Daemonorops draco (RDD) and the resin from Dracaena cochinchinensis (RDC) are equally prescribed as "Dragon's Blood" for facilitating blood circulation. Aim of the study: To verify the traditional efficacy and elucidate the mechanism, the present study compared the chemical profiles and the pharmacological effects of two species of "Dragon's Blood" mainly used in China. Materials and methods: A UPLC-MS fingerprinting method was developed to compare the chemical profiles of the two medicines. The anti-platelet aggregation effects of the two medicines induced by arachidonic acid (AA) were investigated. Results: The chemical profiles of these two species of "Dragon's Blood" were significantly different. The characteristic constituents were found to be: flavanes in RDD and stilbenes in RDC. In the in vivo platelet inhibition test, performed with the dose of 200 mg/kg on rats, the peak inhibitory effects of RDD and RDC were 35.8% and 27.6%, respectively, compared with the control group. With the in vitro concentrations of 0.2, 0.4 and 0.8 mg/ml, RDD exerted significant inhibition of aggregation by 18.7%, 20.0%, and 61.6%, respectively, and RDC exerted significant inhibition of aggregation by 13.3%, 20.2%, and 31.6%, respectively.Conclusion: The fingerprinting method used here is suitable for distinguishing them. All pharmacological tests indicated that RDD was more potent than RDC against platelet aggregation.
AB - Ethnopharmacological relevance: "Dragon's Blood" has been used as a medicine since ancient times by many cultures. In traditional Chinese medicine, the resin obtained from Daemonorops draco (RDD) and the resin from Dracaena cochinchinensis (RDC) are equally prescribed as "Dragon's Blood" for facilitating blood circulation. Aim of the study: To verify the traditional efficacy and elucidate the mechanism, the present study compared the chemical profiles and the pharmacological effects of two species of "Dragon's Blood" mainly used in China. Materials and methods: A UPLC-MS fingerprinting method was developed to compare the chemical profiles of the two medicines. The anti-platelet aggregation effects of the two medicines induced by arachidonic acid (AA) were investigated. Results: The chemical profiles of these two species of "Dragon's Blood" were significantly different. The characteristic constituents were found to be: flavanes in RDD and stilbenes in RDC. In the in vivo platelet inhibition test, performed with the dose of 200 mg/kg on rats, the peak inhibitory effects of RDD and RDC were 35.8% and 27.6%, respectively, compared with the control group. With the in vitro concentrations of 0.2, 0.4 and 0.8 mg/ml, RDD exerted significant inhibition of aggregation by 18.7%, 20.0%, and 61.6%, respectively, and RDC exerted significant inhibition of aggregation by 13.3%, 20.2%, and 31.6%, respectively.Conclusion: The fingerprinting method used here is suitable for distinguishing them. All pharmacological tests indicated that RDD was more potent than RDC against platelet aggregation.
KW - Anti-platelet aggregation
KW - Chemical profiles
KW - Daemonorops draco
KW - Dracaena cochinchinensis
KW - Dragon's Blood
UR - http://www.scopus.com/inward/record.url?scp=78651388253&partnerID=8YFLogxK
U2 - 10.1016/j.jep.2010.11.008
DO - 10.1016/j.jep.2010.11.008
M3 - Journal article
C2 - 21073937
AN - SCOPUS:78651388253
SN - 0378-8741
VL - 133
SP - 796
EP - 802
JO - Journal of Ethnopharmacology
JF - Journal of Ethnopharmacology
IS - 2
ER -