Comparison of contents of five ephedrine alkaloids in three official origins of Ephedra Herb in China by high-performance liquid chromatography

Hao Hong; Hubiao CHEN; Dong Hui Yang; Ming Ying Shang; Xuan Wang; Shao Qing Cai; Masayuki Mikage

doi:10.1007/s11418-011-0528-8

Comparison of contents of five ephedrine alkaloids in three official origins of Ephedra Herb in China by high-performance liquid chromatography

Hao Hong, Hubiao CHEN, Dong Hui Yang, Ming Ying Shang, Xuan Wang, Shao Qing Cai^*, Masayuki Mikage

^*Corresponding author for this work

School of Chinese Medicine

Research output: Contribution to journal › Article › peer-review

35 Citations (Scopus)

Abstract

In the present paper, a fast and economical HPLC method [on a Phenomenex Polar-RP column with a solution of (phosphoric acid:triethylamine:dibutylamine: water = 0.40:0.1:0.2:499.3) and methanol] is developed, and applied for the determination of norephedrine, norpseudoephedrine, ephedrine (E), pseudoephedrine (PE) and methylephedrine (ME) in 64 samples of three species from main habitats in China. Quantitation data showed that total alkaloid content in Ephedra equisetina Bge. (2.708 ± 0.642%) is higher than that in E. sinica Stapf. (1.365 ± 0.624%) and E. intermedia Schrenk et C. A. Mey. (1.537 ± 0.746%), but the range of total alkaloid content in each species is so wide that the ranges of the three species greatly overlap. The contents of E, PE and ME are different among the three species. The ratio E/total alkaloid content and ratio E/PE as well as E and ME contents can be used for identification of E. sinica, E. intermedia and E. equisetina from one another.

Original language	English
Pages (from-to)	623-628
Number of pages	6
Journal	Journal of Natural Medicines
Volume	65
Issue number	3-4
DOIs	https://doi.org/10.1007/s11418-011-0528-8
Publication status	Published - Jul 2011

Scopus Subject Areas

Pharmaceutical Science
Drug Discovery
Complementary and alternative medicine
Organic Chemistry

User-Defined Keywords

Ephedra alkaloids
Ephedra Herb
Mahuang
Simultaneous determination
Species identification

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10.1007/s11418-011-0528-8

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title = "Comparison of contents of five ephedrine alkaloids in three official origins of Ephedra Herb in China by high-performance liquid chromatography",

abstract = "In the present paper, a fast and economical HPLC method [on a Phenomenex Polar-RP column with a solution of (phosphoric acid:triethylamine:dibutylamine: water = 0.40:0.1:0.2:499.3) and methanol] is developed, and applied for the determination of norephedrine, norpseudoephedrine, ephedrine (E), pseudoephedrine (PE) and methylephedrine (ME) in 64 samples of three species from main habitats in China. Quantitation data showed that total alkaloid content in Ephedra equisetina Bge. (2.708 ± 0.642%) is higher than that in E. sinica Stapf. (1.365 ± 0.624%) and E. intermedia Schrenk et C. A. Mey. (1.537 ± 0.746%), but the range of total alkaloid content in each species is so wide that the ranges of the three species greatly overlap. The contents of E, PE and ME are different among the three species. The ratio E/total alkaloid content and ratio E/PE as well as E and ME contents can be used for identification of E. sinica, E. intermedia and E. equisetina from one another.",

keywords = "Ephedra alkaloids, Ephedra Herb, Mahuang, Simultaneous determination, Species identification",

author = "Hao Hong and Hubiao CHEN and Yang, {Dong Hui} and Shang, {Ming Ying} and Xuan Wang and Cai, {Shao Qing} and Masayuki Mikage",

note = "Funding Information: Acknowledgments The work was financially supported by the National Outstanding Youth Funds of China (grant no. 30425018).",

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doi = "10.1007/s11418-011-0528-8",

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T1 - Comparison of contents of five ephedrine alkaloids in three official origins of Ephedra Herb in China by high-performance liquid chromatography

AU - Hong, Hao

AU - CHEN, Hubiao

AU - Yang, Dong Hui

AU - Shang, Ming Ying

AU - Wang, Xuan

AU - Cai, Shao Qing

AU - Mikage, Masayuki

N1 - Funding Information: Acknowledgments The work was financially supported by the National Outstanding Youth Funds of China (grant no. 30425018).

PY - 2011/7

Y1 - 2011/7

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AB - In the present paper, a fast and economical HPLC method [on a Phenomenex Polar-RP column with a solution of (phosphoric acid:triethylamine:dibutylamine: water = 0.40:0.1:0.2:499.3) and methanol] is developed, and applied for the determination of norephedrine, norpseudoephedrine, ephedrine (E), pseudoephedrine (PE) and methylephedrine (ME) in 64 samples of three species from main habitats in China. Quantitation data showed that total alkaloid content in Ephedra equisetina Bge. (2.708 ± 0.642%) is higher than that in E. sinica Stapf. (1.365 ± 0.624%) and E. intermedia Schrenk et C. A. Mey. (1.537 ± 0.746%), but the range of total alkaloid content in each species is so wide that the ranges of the three species greatly overlap. The contents of E, PE and ME are different among the three species. The ratio E/total alkaloid content and ratio E/PE as well as E and ME contents can be used for identification of E. sinica, E. intermedia and E. equisetina from one another.

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Comparison of contents of five ephedrine alkaloids in three official origins of Ephedra Herb in China by high-performance liquid chromatography

Abstract

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