TY - JOUR
T1 - Comparison of contents of five ephedrine alkaloids in three official origins of Ephedra Herb in China by high-performance liquid chromatography
AU - Hong, Hao
AU - Chen, Hubiao
AU - Yang, Dong Hui
AU - Shang, Ming Ying
AU - Wang, Xuan
AU - Cai, Shao Qing
AU - Mikage, Masayuki
N1 - Funding Information:
Acknowledgments The work was financially supported by the National Outstanding Youth Funds of China (grant no. 30425018).
PY - 2011/7
Y1 - 2011/7
N2 - In the present paper, a fast and economical HPLC method [on a Phenomenex Polar-RP column with a solution of (phosphoric acid:triethylamine:dibutylamine: water = 0.40:0.1:0.2:499.3) and methanol] is developed, and applied for the determination of norephedrine, norpseudoephedrine, ephedrine (E), pseudoephedrine (PE) and methylephedrine (ME) in 64 samples of three species from main habitats in China. Quantitation data showed that total alkaloid content in Ephedra equisetina Bge. (2.708 ± 0.642%) is higher than that in E. sinica Stapf. (1.365 ± 0.624%) and E. intermedia Schrenk et C. A. Mey. (1.537 ± 0.746%), but the range of total alkaloid content in each species is so wide that the ranges of the three species greatly overlap. The contents of E, PE and ME are different among the three species. The ratio E/total alkaloid content and ratio E/PE as well as E and ME contents can be used for identification of E. sinica, E. intermedia and E. equisetina from one another.
AB - In the present paper, a fast and economical HPLC method [on a Phenomenex Polar-RP column with a solution of (phosphoric acid:triethylamine:dibutylamine: water = 0.40:0.1:0.2:499.3) and methanol] is developed, and applied for the determination of norephedrine, norpseudoephedrine, ephedrine (E), pseudoephedrine (PE) and methylephedrine (ME) in 64 samples of three species from main habitats in China. Quantitation data showed that total alkaloid content in Ephedra equisetina Bge. (2.708 ± 0.642%) is higher than that in E. sinica Stapf. (1.365 ± 0.624%) and E. intermedia Schrenk et C. A. Mey. (1.537 ± 0.746%), but the range of total alkaloid content in each species is so wide that the ranges of the three species greatly overlap. The contents of E, PE and ME are different among the three species. The ratio E/total alkaloid content and ratio E/PE as well as E and ME contents can be used for identification of E. sinica, E. intermedia and E. equisetina from one another.
KW - Ephedra alkaloids
KW - Ephedra Herb
KW - Mahuang
KW - Simultaneous determination
KW - Species identification
UR - http://www.scopus.com/inward/record.url?scp=79960453382&partnerID=8YFLogxK
U2 - 10.1007/s11418-011-0528-8
DO - 10.1007/s11418-011-0528-8
M3 - Journal article
C2 - 21465337
AN - SCOPUS:79960453382
SN - 1340-3443
VL - 65
SP - 623
EP - 628
JO - Journal of Natural Medicines
JF - Journal of Natural Medicines
IS - 3-4
ER -