Comparative study of chemical and enzymatic pre-treatments of Kitchen waste (KW) to generate fermentable sugars for the production of polyhydroxyalkanoates (PHA)

Shraddha Chavan, Bhoomika Yadav, R. D. Tyagi*, Jonathan W.C. Wong, Patrick Drogui

*Corresponding author for this work

Research output: Contribution to journalJournal articlepeer-review

4 Citations (Scopus)

Abstract

Kitchen waste (KW) production is anticipated to rise because of accelerated urban and economic growth, particularly in Asian nations. Therefore, applications/recycling of KW should be explored for its effective utilization to generate a sustainable source of energy that will ultimately avoid massive economic and energy losses. The main goal of this study was to investigate the combinational effect of thermochemical and enzymatic pre-treatment on synthetic KW to enhance the release of fermentable sugars for the synthesis of value-added products such as polyhydroxyalkanoates (PHAs). The thermochemical pre-treatment of KW samples was performed using 4 N HCl, 4 N H2SO4, and 4 N NaOH at a concentration of 0–30% (v/v), 30–100 °C for 0–120 min. Furthermore, to enhance the total fermentable sugars, variable enzyme dosages were added after acidic or alkaline pre-treatments (0–1% v/v α-amylase and 0–1% v/v amyloglucosidase-AMG) while maintaining a constant pH 5, temperature 50 °C, and time 30 min. Pre-treatment of KW with acid (15% v/v 4 N HCl in water) at temperature 100 °C, followed by enzymatic hydrolysis, i.e., 0.6% (v/v) α-amylase and 0.4% (v/v) amyloglucosidase (at pH 5, 50 0C and 60 min) resulted in the maximum concentration of fermentable reducing sugars (46.13 g/L) in the hydrolysate.

Original languageEnglish
Article number109240
JournalBiochemical Engineering Journal
Volume204
DOIs
Publication statusPublished - Apr 2024

Scopus Subject Areas

  • Biotechnology
  • Bioengineering
  • Environmental Engineering
  • Biomedical Engineering

User-Defined Keywords

  • Kitchen waste
  • Pre-treatment
  • Fermentation
  • Bioplastics
  • Amylase enzyme
  • Amyloglucosidase
  • Reducing sugars

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