TY - JOUR
T1 - Comparative study of chemical and enzymatic pre-treatments of Kitchen waste (KW) to generate fermentable sugars for the production of polyhydroxyalkanoates (PHA)
AU - Chavan, Shraddha
AU - Yadav, Bhoomika
AU - Tyagi, R. D.
AU - Wong, Jonathan W.C.
AU - Drogui, Patrick
N1 - This research did not receive any specific grant from funding agencies in the public, commercial, or not-for-profit sectors.
Publisher Copyright:
© 2024 Elsevier B.V.
PY - 2024/4
Y1 - 2024/4
N2 - Kitchen waste (KW) production is anticipated to rise because of accelerated urban and economic growth, particularly in Asian nations. Therefore, applications/recycling of KW should be explored for its effective utilization to generate a sustainable source of energy that will ultimately avoid massive economic and energy losses. The main goal of this study was to investigate the combinational effect of thermochemical and enzymatic pre-treatment on synthetic KW to enhance the release of fermentable sugars for the synthesis of value-added products such as polyhydroxyalkanoates (PHAs). The thermochemical pre-treatment of KW samples was performed using 4 N HCl, 4 N H2SO4, and 4 N NaOH at a concentration of 0–30% (v/v), 30–100 °C for 0–120 min. Furthermore, to enhance the total fermentable sugars, variable enzyme dosages were added after acidic or alkaline pre-treatments (0–1% v/v α-amylase and 0–1% v/v amyloglucosidase-AMG) while maintaining a constant pH 5, temperature 50 °C, and time 30 min. Pre-treatment of KW with acid (15% v/v 4 N HCl in water) at temperature 100 °C, followed by enzymatic hydrolysis, i.e., 0.6% (v/v) α-amylase and 0.4% (v/v) amyloglucosidase (at pH 5, 50 0C and 60 min) resulted in the maximum concentration of fermentable reducing sugars (46.13 g/L) in the hydrolysate.
AB - Kitchen waste (KW) production is anticipated to rise because of accelerated urban and economic growth, particularly in Asian nations. Therefore, applications/recycling of KW should be explored for its effective utilization to generate a sustainable source of energy that will ultimately avoid massive economic and energy losses. The main goal of this study was to investigate the combinational effect of thermochemical and enzymatic pre-treatment on synthetic KW to enhance the release of fermentable sugars for the synthesis of value-added products such as polyhydroxyalkanoates (PHAs). The thermochemical pre-treatment of KW samples was performed using 4 N HCl, 4 N H2SO4, and 4 N NaOH at a concentration of 0–30% (v/v), 30–100 °C for 0–120 min. Furthermore, to enhance the total fermentable sugars, variable enzyme dosages were added after acidic or alkaline pre-treatments (0–1% v/v α-amylase and 0–1% v/v amyloglucosidase-AMG) while maintaining a constant pH 5, temperature 50 °C, and time 30 min. Pre-treatment of KW with acid (15% v/v 4 N HCl in water) at temperature 100 °C, followed by enzymatic hydrolysis, i.e., 0.6% (v/v) α-amylase and 0.4% (v/v) amyloglucosidase (at pH 5, 50 0C and 60 min) resulted in the maximum concentration of fermentable reducing sugars (46.13 g/L) in the hydrolysate.
KW - Kitchen waste
KW - Pre-treatment
KW - Fermentation
KW - Bioplastics
KW - Amylase enzyme
KW - Amyloglucosidase
KW - Reducing sugars
UR - http://www.scopus.com/inward/record.url?scp=85184776943&partnerID=8YFLogxK
U2 - 10.1016/j.bej.2024.109240
DO - 10.1016/j.bej.2024.109240
M3 - Journal article
AN - SCOPUS:85184776943
SN - 1369-703X
VL - 204
JO - Biochemical Engineering Journal
JF - Biochemical Engineering Journal
M1 - 109240
ER -