Comparative metabolic profiling reveals secondary metabolites correlated with soybean salt tolerance

Wei Wu; Qing Zhang; Yanming Zhu; Hon Ming Lam; Zongwei CAI; Dianjing Guo

doi:10.1021/jf8024024

Comparative metabolic profiling reveals secondary metabolites correlated with soybean salt tolerance

Wei Wu, Qing Zhang, Yanming Zhu, Hon Ming Lam, Zongwei CAI, Dianjing Guo^*

^*Corresponding author for this work

Department of Chemistry

Research output: Contribution to journal › Journal article › peer-review

55 Citations (Scopus)

Abstract

High-performance liquid chromatography-ultraviolet-electrospray ionization mass spectrometry (HPLC-UV-ESI-MS) and HPLC-ESI-MSⁿ analysis methods were used for metabolic profiling and simultaneous identification of isoflavonoids and saponins in soybean seeds. Comparative targeted metabolic profiling revealed marked differences in the metabolite composition between salt-sensitive and salt-tolerant soybean varieties. Principle component analysis clearly demonstrated that it is possible to use secondary metabolites, for example, isoflavones and saponins, to discriminate between closely related soybean genotypes. Genistin and group B saponins were identified as the key secondary metabolites correlated with salt tolerance. These individual metabolites may provide additional insight into the salt tolerance and adaptation of plants.

Original language	English
Pages (from-to)	11132-11138
Number of pages	7
Journal	Journal of Agricultural and Food Chemistry
Volume	56
Issue number	23
DOIs	https://doi.org/10.1021/jf8024024
Publication status	Published - 10 Dec 2008

Scopus Subject Areas

Chemistry(all)
Agricultural and Biological Sciences(all)

User-Defined Keywords

HPLC-ESI-MS
Isoflavonoid
Metabolic profiling
Soyasaponin
Soybean

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10.1021/jf8024024

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title = "Comparative metabolic profiling reveals secondary metabolites correlated with soybean salt tolerance",

abstract = "High-performance liquid chromatography-ultraviolet-electrospray ionization mass spectrometry (HPLC-UV-ESI-MS) and HPLC-ESI-MSn analysis methods were used for metabolic profiling and simultaneous identification of isoflavonoids and saponins in soybean seeds. Comparative targeted metabolic profiling revealed marked differences in the metabolite composition between salt-sensitive and salt-tolerant soybean varieties. Principle component analysis clearly demonstrated that it is possible to use secondary metabolites, for example, isoflavones and saponins, to discriminate between closely related soybean genotypes. Genistin and group B saponins were identified as the key secondary metabolites correlated with salt tolerance. These individual metabolites may provide additional insight into the salt tolerance and adaptation of plants.",

keywords = "HPLC-ESI-MS, Isoflavonoid, Metabolic profiling, Soyasaponin, Soybean",

author = "Wei Wu and Qing Zhang and Yanming Zhu and Lam, {Hon Ming} and Zongwei CAI and Dianjing Guo",

note = "Hong Kong UGC AoE Plant & Agricultural Biotechnology Project AoE-B-07/09 and the Institute of Plant Molecular Biology and Agrobiotechnology at The Chinese University of Hong Kong. ",

year = "2008",

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doi = "10.1021/jf8024024",

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journal = "Journal of Agricultural and Food Chemistry",

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T1 - Comparative metabolic profiling reveals secondary metabolites correlated with soybean salt tolerance

AU - Wu, Wei

AU - Zhang, Qing

AU - Zhu, Yanming

AU - Lam, Hon Ming

AU - CAI, Zongwei

AU - Guo, Dianjing

N1 - Hong Kong UGC AoE Plant & Agricultural Biotechnology Project AoE-B-07/09 and the Institute of Plant Molecular Biology and Agrobiotechnology at The Chinese University of Hong Kong.

PY - 2008/12/10

Y1 - 2008/12/10

N2 - High-performance liquid chromatography-ultraviolet-electrospray ionization mass spectrometry (HPLC-UV-ESI-MS) and HPLC-ESI-MSn analysis methods were used for metabolic profiling and simultaneous identification of isoflavonoids and saponins in soybean seeds. Comparative targeted metabolic profiling revealed marked differences in the metabolite composition between salt-sensitive and salt-tolerant soybean varieties. Principle component analysis clearly demonstrated that it is possible to use secondary metabolites, for example, isoflavones and saponins, to discriminate between closely related soybean genotypes. Genistin and group B saponins were identified as the key secondary metabolites correlated with salt tolerance. These individual metabolites may provide additional insight into the salt tolerance and adaptation of plants.

AB - High-performance liquid chromatography-ultraviolet-electrospray ionization mass spectrometry (HPLC-UV-ESI-MS) and HPLC-ESI-MSn analysis methods were used for metabolic profiling and simultaneous identification of isoflavonoids and saponins in soybean seeds. Comparative targeted metabolic profiling revealed marked differences in the metabolite composition between salt-sensitive and salt-tolerant soybean varieties. Principle component analysis clearly demonstrated that it is possible to use secondary metabolites, for example, isoflavones and saponins, to discriminate between closely related soybean genotypes. Genistin and group B saponins were identified as the key secondary metabolites correlated with salt tolerance. These individual metabolites may provide additional insight into the salt tolerance and adaptation of plants.

KW - HPLC-ESI-MS

KW - Isoflavonoid

KW - Metabolic profiling

KW - Soyasaponin

KW - Soybean

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JO - Journal of Agricultural and Food Chemistry

JF - Journal of Agricultural and Food Chemistry

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ER -

Comparative metabolic profiling reveals secondary metabolites correlated with soybean salt tolerance

Abstract

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