TY - JOUR
T1 - Comparative authentication of three "snow lotus" herbs by macroscopic and microscopic features
AU - Chen, Qilei
AU - Yi, Tao
AU - Tang, Yina
AU - Wong, Lai Lai
AU - Huang, Xiaoxuan
AU - Zhao, Zhongzhen
AU - Chen, Hubiao
N1 - Contract grant sponsor: Faculty Research Grant of Hong Kong Baptist University; Contract grant number: FRG/08–09/II-52; Contract grant sponsor: General Research Fund of Hong Kong; Contract grant number: HKBU-260111.
PY - 2014/8
Y1 - 2014/8
N2 - "Snow lotus" is a famous Chinese Materia Medica derived from species of the genus Saussurea (Compositae). To differentiate three representative easily-confused snow lotus herbs, namely, Saussurea involucrata (Kar. et Kir.) Sch.-Bip, Saussurea laniceps Hand.-Mazz., and Saussurea medusa Maxim., macroscopic features of the three herbs were systemically observed, and microscopic features were compared by using ordinary light microscopy, polarized light microscopy and scanning electron microscopy (SEM). The results indicate that, as for macroscopic identification, capitula situation and arrangement, and as for microscopic identification, pollen grains, nonglandular hairs, glandular hairs, and cells of inner surface of the microdiodange can be used to authenticate the three snow lotus herbs. Comprehensive table comparing the characteristics were presented in this study. SEM has been found to provide a number of unique characteristics of pollen grains. Based on the observation of pollen grains, evolution sequence of the three species was speculated. The present method was proven to be efficient, convenient, simple, and reliable, which was successfully applied to the authentication of three snow lotus herbs.
AB - "Snow lotus" is a famous Chinese Materia Medica derived from species of the genus Saussurea (Compositae). To differentiate three representative easily-confused snow lotus herbs, namely, Saussurea involucrata (Kar. et Kir.) Sch.-Bip, Saussurea laniceps Hand.-Mazz., and Saussurea medusa Maxim., macroscopic features of the three herbs were systemically observed, and microscopic features were compared by using ordinary light microscopy, polarized light microscopy and scanning electron microscopy (SEM). The results indicate that, as for macroscopic identification, capitula situation and arrangement, and as for microscopic identification, pollen grains, nonglandular hairs, glandular hairs, and cells of inner surface of the microdiodange can be used to authenticate the three snow lotus herbs. Comprehensive table comparing the characteristics were presented in this study. SEM has been found to provide a number of unique characteristics of pollen grains. Based on the observation of pollen grains, evolution sequence of the three species was speculated. The present method was proven to be efficient, convenient, simple, and reliable, which was successfully applied to the authentication of three snow lotus herbs.
KW - snow lotus
KW - Saussurea
KW - ordinary light microscopy
KW - polarized light microscopy
KW - scanning electron microscopy
UR - http://www.scopus.com/inward/record.url?scp=84904507074&partnerID=8YFLogxK
U2 - 10.1002/jemt.22381
DO - 10.1002/jemt.22381
M3 - Journal article
C2 - 24841997
AN - SCOPUS:84904507074
SN - 1059-910X
VL - 77
SP - 631
EP - 641
JO - Microscopy Research and Technique
JF - Microscopy Research and Technique
IS - 8
ER -