Comparative analysis of Oldenlandia diffusa and its substitutes by high performance liquid chromatographic fingerprint and mass spectrometric analysis

Zhitao LIANG, Zhi Hong JIANG, Hingman Ho, Zhongzhen ZHAO*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

14 Citations (Scopus)

Abstract

Herba Oldenlandiae is the dried whole herb of Oldenlandia diffusa (Willd.) Roxb. (Family Rubiaceae) recorded in the Chinese pharmacopoeia. However, the herbs of O. corymbosa (L.) Lam and O. tenelliflora Bl. are also often available and used indiscriminately as Herba Oldenlandiae in the markets. In light of this, high-performance liquid chromatography (HPLC) combined with high resolution mass spectrometry (HR-MS) has been developed for a comparative analysis of chemical components in the three Oldenlandia species. The correlation coefficients of the entire chromatographic patterns of samples were calculated by software and used for appraising the similarity. The chemical information on the peaks in the chromatograms of O. diffusa was obtained by comparison of their exact mass data, UV spectra and literature values. The results indicated that the chemical profiling of O. diffusa is quite different in samples collected from different habitats. Furthermore, the variations in the abundance of asperuloside, E-6-O-p-coumaroylscandoside methyl ester and E-6-O-p-coumaroylscandoside methyl ester-10-methyl ether were significant in these related herbs. In conclusion, it is important to use the above three compounds as chemical markers for quality evaluation and chemical authentication of Herba Oldenlandiae and its substitutes.

Original languageEnglish
Pages (from-to)1502-1508
Number of pages7
JournalPlanta Medica
Volume73
Issue number14
DOIs
Publication statusPublished - Nov 2007

Scopus Subject Areas

  • Analytical Chemistry
  • Molecular Medicine
  • Pharmacology
  • Pharmaceutical Science
  • Drug Discovery
  • Complementary and alternative medicine
  • Organic Chemistry

User-Defined Keywords

  • Fingerprint
  • High-performance liquid chromatography
  • Oldenlandia diffusa
  • Rubiaceae
  • Substitute

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