TY - JOUR
T1 - Comparative analysis of mammalian stanniocalcin genes
AU - Varghese, Robin
AU - Wong, Chris K. C.
AU - Deol, Harminder
AU - Wagner, Graham F.
AU - Dimattia, Gabriel E.
N1 - Funding Information:
This work was supported by grants from London Health Sciences Research, Inc. (to G.E.D.), the London Regional Cancer Center (to G.E.D.), and the Medical Research Council of Canada (to G.F.W.).
Publisher Copyright:
© 1998 by The Endocrine Society
PY - 1998/11/1
Y1 - 1998/11/1
N2 - The recent discovery of mammalian stanniocalcin (STC) prompted an investigation of its gene structure and expression pattern to study its function and regulation. We show that both the human and mouse genes are composed of four exons spanning about 13 kb, with 85% nucleotide sequence identity in coding regions. Remarkably high sequence conservation between species also exists in the approximately 3-kb 3'-untranslated region. Comparative analysis of the 5'-untranslated region and flanking DNA from the rat and human STC genes showed long stretches of CAG trinucleotide repeats and an additional (CA)25 dinucleotide repeat unique to the rat promoter. An analysis of STC expression in the mouse showed that ovary contained the highest level of messenger RNA, with lower, but detectable, levels in most tissues. In situ hybridization revealed strong, specific hybridization over the thecal-interstitial cells of the ovarian stroma, whereas immunohistochemical analysis indicated that STC was present not only in the stroma, but also in the corpora lutea and oocyte of the developing follicle. Consequently, STC may act as a signaling molecule between the thecal-interstitial cell compartment and the corpus luteum and oocyte, thereby regulating the activity of these structures in some way. These findings suggest that in addition to its role in mineral metabolism, STC has acquired an important function in reproduction during its evolution to mammals.
AB - The recent discovery of mammalian stanniocalcin (STC) prompted an investigation of its gene structure and expression pattern to study its function and regulation. We show that both the human and mouse genes are composed of four exons spanning about 13 kb, with 85% nucleotide sequence identity in coding regions. Remarkably high sequence conservation between species also exists in the approximately 3-kb 3'-untranslated region. Comparative analysis of the 5'-untranslated region and flanking DNA from the rat and human STC genes showed long stretches of CAG trinucleotide repeats and an additional (CA)25 dinucleotide repeat unique to the rat promoter. An analysis of STC expression in the mouse showed that ovary contained the highest level of messenger RNA, with lower, but detectable, levels in most tissues. In situ hybridization revealed strong, specific hybridization over the thecal-interstitial cells of the ovarian stroma, whereas immunohistochemical analysis indicated that STC was present not only in the stroma, but also in the corpora lutea and oocyte of the developing follicle. Consequently, STC may act as a signaling molecule between the thecal-interstitial cell compartment and the corpus luteum and oocyte, thereby regulating the activity of these structures in some way. These findings suggest that in addition to its role in mineral metabolism, STC has acquired an important function in reproduction during its evolution to mammals.
UR - http://www.scopus.com/inward/record.url?scp=0031766975&partnerID=8YFLogxK
U2 - 10.1210/endo.139.11.6313
DO - 10.1210/endo.139.11.6313
M3 - Journal article
C2 - 9794484
AN - SCOPUS:0031766975
SN - 0013-7227
VL - 139
SP - 4714
EP - 4725
JO - Endocrinology
JF - Endocrinology
IS - 11
ER -