Comparative analysis of diosgenin in Dioscorea species and related medicinal plants by UPLC-DAD-MS

Tao Yi, Hau Man Suen, Yi Na Tang, Z. Zhao, Hubiao Chen

Research output: Contribution to conferencePaper

Abstract

Background: Dioscorea is a genus of flowering plants, and some Dioscorea species are known and used as a source for the steroidal sapogenin diosgenin. To screen potential resource from Dioscorea species and related medicinal plants for diosgenin extraction, a rapid method to compare the contents of diosgenin in various plants is crucial. Results: An ultra-performance liquid chromatography (UPLC) coupled with diode array detection (DAD) and electrospray ionization mass spectrometry (ESI-MS) method was developed for identification and determination of diosgenin in various plants. A comprehensive validation of the developed method was conducted. Twenty-four batches of plant samples from four Dioscorea species, one Smilax species and two Heterosmilax species were analyzed by using the developed method.The present method presented good sensitivity, precision and accuracy. Diosgenin was found in three Dioscorea species and one Heterosmilax species, namely D. zingiberensis, D. septemloba, D. collettii and H. yunnanensis. Conclusion: The method is suitable for the screening of diosgenin resources from plants. D. zingiberensis is an important resource for diosgenin harvesting.
Original languageEnglish
Publication statusSubmitted - Aug 2014
EventThe 13th Meeting of the Consortium for Globalization of Chinese Medicine (CGCM) = 第十三届中药全球化联盟研讨会 - Beijing, China
Duration: 27 Aug 201429 Aug 2014

Conference

ConferenceThe 13th Meeting of the Consortium for Globalization of Chinese Medicine (CGCM) = 第十三届中药全球化联盟研讨会
Period27/08/1429/08/14

User-Defined Keywords

  • Dioscorea
  • Diosgenin
  • Medicinal plants
  • Quality evaluation
  • UPLC-DAD-MS

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