Clustering of the group III metabotropic glutamate receptor 4a at parallel fiber synaptic terminals in the rat cerebellar molecular layer

José María Mateos; Izaskun Elezgarai; Rocío Benítez; Alexandra Osorio; Aurora Bilbao; Jon Jatsu Azkue; Rainer Kuhn; Thomas Knöpfel; Pedro Grandes

doi:10.1016/S0168-0102(99)00066-8

Clustering of the group III metabotropic glutamate receptor 4a at parallel fiber synaptic terminals in the rat cerebellar molecular layer

José María Mateos
, Izaskun Elezgarai
, Rocío Benítez
, Alexandra Osorio
, Aurora Bilbao
, Jon Jatsu Azkue
, Rainer Kuhn
, Thomas Knöpfel
, Pedro Grandes^*

^*Corresponding author for this work

Department of Physics

Research output: Contribution to journal › Journal article › peer-review

31 Citations (Scopus)

Abstract

We report in this study with a pre-embedding immunogold method, the clustering of the group III metabotropic glutamate receptor 4a (mGluR4a) along the presynaptic membrane of parallel fiber synaptic terminals in the cerebellar molecular layer. The mGluR4a clusters were homogeneously distributed and interspaced by about 60 nm. These results suggest a particular arrangement of mGluR4a which might help to a rapid and effective activation of this receptor by glutamate.

Original language	English
Pages (from-to)	71-74
Number of pages	4
Journal	Neuroscience Research
Volume	35
Issue number	1
DOIs	https://doi.org/10.1016/S0168-0102(99)00066-8
Publication status	Published - Oct 1999

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User-Defined Keywords

Cerebellum
Electron microscopy
Excitatory neurotransmission
G-proteins
Immunogold
Pre- embedding immunocytochemistry

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10.1016/S0168-0102(99)00066-8

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title = "Clustering of the group III metabotropic glutamate receptor 4a at parallel fiber synaptic terminals in the rat cerebellar molecular layer",

abstract = "We report in this study with a pre-embedding immunogold method, the clustering of the group III metabotropic glutamate receptor 4a (mGluR4a) along the presynaptic membrane of parallel fiber synaptic terminals in the cerebellar molecular layer. The mGluR4a clusters were homogeneously distributed and interspaced by about 60 nm. These results suggest a particular arrangement of mGluR4a which might help to a rapid and effective activation of this receptor by glutamate.",

keywords = "Cerebellum, Electron microscopy, Excitatory neurotransmission, G-proteins, Immunogold, Pre- embedding immunocytochemistry",

author = "Mateos, \{Jos{\'e} Mar{\'i}a\} and Izaskun Elezgarai and Roc{\'i}o Ben{\'i}tez and Alexandra Osorio and Aurora Bilbao and Azkue, \{Jon Jatsu\} and Rainer Kuhn and Thomas Kn{\"o}pfel and Pedro Grandes",

note = "We thank D. R{\"u}egg for excellent help in the generation and purification of the antiserum. Part of this work has been supported by Ministerio de Educaci{\'o}n y Cultura DGICYT grant PB95-0347 and Gobierno Vasco grant PI-1997-41. J.M. Mateos; J.J. Azkue and A. Osorio were in receipt of fellowships from Gobierno Vasco (grants BFI 95.154, BFI98.18, BFI98.109, respectively) and R. Ben{\'ı}tez and I. Elezgarai from the Basque Country University (1996 and 1998, respectively). Publisher copyright: {\textcopyright} 1999 Elsevier Science Ireland Ltd. All rights reserved.",

year = "1999",

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doi = "10.1016/S0168-0102(99)00066-8",

language = "English",

volume = "35",

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T1 - Clustering of the group III metabotropic glutamate receptor 4a at parallel fiber synaptic terminals in the rat cerebellar molecular layer

AU - Mateos, José María

AU - Elezgarai, Izaskun

AU - Benítez, Rocío

AU - Osorio, Alexandra

AU - Bilbao, Aurora

AU - Azkue, Jon Jatsu

AU - Kuhn, Rainer

AU - Knöpfel, Thomas

AU - Grandes, Pedro

N1 - We thank D. Rüegg for excellent help in the generation and purification of the antiserum. Part of this work has been supported by Ministerio de Educación y Cultura DGICYT grant PB95-0347 and Gobierno Vasco grant PI-1997-41. J.M. Mateos; J.J. Azkue and A. Osorio were in receipt of fellowships from Gobierno Vasco (grants BFI 95.154, BFI98.18, BFI98.109, respectively) and R. Benı́tez and I. Elezgarai from the Basque Country University (1996 and 1998, respectively). Publisher copyright: © 1999 Elsevier Science Ireland Ltd. All rights reserved.

PY - 1999/10

Y1 - 1999/10

N2 - We report in this study with a pre-embedding immunogold method, the clustering of the group III metabotropic glutamate receptor 4a (mGluR4a) along the presynaptic membrane of parallel fiber synaptic terminals in the cerebellar molecular layer. The mGluR4a clusters were homogeneously distributed and interspaced by about 60 nm. These results suggest a particular arrangement of mGluR4a which might help to a rapid and effective activation of this receptor by glutamate.

AB - We report in this study with a pre-embedding immunogold method, the clustering of the group III metabotropic glutamate receptor 4a (mGluR4a) along the presynaptic membrane of parallel fiber synaptic terminals in the cerebellar molecular layer. The mGluR4a clusters were homogeneously distributed and interspaced by about 60 nm. These results suggest a particular arrangement of mGluR4a which might help to a rapid and effective activation of this receptor by glutamate.

KW - Cerebellum

KW - Electron microscopy

KW - Excitatory neurotransmission

KW - G-proteins

KW - Immunogold

KW - Pre- embedding immunocytochemistry

UR - https://www.scopus.com/pages/publications/0032845643

U2 - 10.1016/S0168-0102(99)00066-8

DO - 10.1016/S0168-0102(99)00066-8

M3 - Journal article

C2 - 10555166

AN - SCOPUS:0032845643

SN - 0168-0102

VL - 35

SP - 71

EP - 74

JO - Neuroscience Research

JF - Neuroscience Research

IS - 1

ER -

Clustering of the group III metabotropic glutamate receptor 4a at parallel fiber synaptic terminals in the rat cerebellar molecular layer

Abstract

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