Cloning and characterization of the promoter region of the mouse frizzled-related protein 4 gene

Vincent Kam Wai Wong, Judy Wai Ping Yam, Wendy W L HSIAO*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)

Abstract

Frizzled-related protein (Frp) is a newly identified family of secreted proteins involved in the Wnt signaling pathway. To date, little is known about the underlying mechanisms regulating Frp expression. In this study the promoter region of mouse frizzled related protein 4 (sFrp4) gene was cloned, sequenced, and analyzed using transient reporter assays along with site-directed mutagenesis. Two clusters of cis-acting elements, STAT3/Lyf-1/MZF1 (site 1) and C/EBP-β/ GATA-1/CREB (site 2) located in the promoter region from - 238 to - 144 were found to be essential for the promoter activity of sFrp4. In addition to sites 1 and 2, putative transcriptional factor binding sites for TFIID, SP1/GC and ATF/CREB exhibited positive, while the site for NRSE exhibited negative regulatory functions, as determined by the alkaline phosphatase activities of the reporter assay. We also demonstrate that the ATF/CREB site may cooperatively interact with the NRSF-like element in regulating sFrp4 promoter activity. The data of our study, which is the first promoter analysis of mouse Frp genes, provide the basis for understanding the functions and the regulation of Frp and its role in regulating Wnt signals.

Original languageEnglish
Pages (from-to)1147-1154
Number of pages8
JournalBiological Chemistry
Volume384
Issue number8
DOIs
Publication statusPublished - 1 Aug 2003

Scopus Subject Areas

  • Biochemistry
  • Molecular Biology
  • Clinical Biochemistry

User-Defined Keywords

  • cAMP responsive element binding protein
  • Frizzled-related protein
  • Neuron-restrictive silencer element

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