Clinical determination of glucose in human serum by a tomato skin biosensor

Hui Han, Yi Li, Huan Yue, Zaide Zhou, Dan Xiao*, Martin M F CHOI

*Corresponding author for this work

Research output: Contribution to journalJournal articlepeer-review

17 Citations (Scopus)


Background: Glucose biosensors based on enzyme reaction of glucose oxidase were studied because the symptomatic therapy of diabetes mellitus requires reliable assessment of blood glucose level at frequent intervals. Tomato skin membranes have been successfully employed to entrap glucose oxidase for fabrication of glucose biosensor. Methods: Glucose oxidase was immobilized onto the tomato skin and the enzyme membrane was then positioned on the surface of an oxygen electrode. The glucose concentration was quantified by the change of dissolved oxygen. All the serum samples were also simultaneously determined by a Hitachi 7060 chemistry analyzer. Results: The response of the biosensor showed a linear relationship with a concentration range of 1.0-30.0 mmol/l glucose. The limit of detection was 0.20 mmol/l. Error Grid analysis demonstrated that 100% of the results fell within clinically acceptable zones A and B. The F- and t-tests showed no significant differences between the 2 methods. The recovery was 95.0-110.0% for 30 serum samples analysis. Conclusions: The tomato skin biosensor possesses the advantages of simple fabrication, fast response time, low cost and high sensitivity. The results of our method are more accurate than and match well with the current clinical instrument method.

Original languageEnglish
Pages (from-to)155-158
Number of pages4
JournalClinica Chimica Acta
Issue number1-2
Publication statusPublished - Sept 2008

Scopus Subject Areas

  • Biochemistry
  • Clinical Biochemistry
  • Biochemistry, medical

User-Defined Keywords

  • Accuracy
  • Blood glucose
  • Error Grid analysis
  • Simplicity
  • Tomato skin biosensor


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