TY - JOUR
T1 - Citrate-coated magnetic polyethyleneimine composites for plasmid DNA delivery into Glioblastoma
AU - Leung, Ken Cham Fai
AU - Sham, Kathy W.Y.
AU - Lai, Josie M.Y.
AU - Wang, Yi Xiang J.
AU - Wong, Chi Hin
AU - Cheng, Christopher H.K.
N1 - Funding Information:
Funding: We acknowledge the financial support partially by The Chinese University of Hong Kong as well as The Hong Kong Baptist University (SKLP_1920_P05, RC-IRCMs/17-18/03 and RC-KRPS-20-21/02).
Publisher Copyright:
© 2021 by the authors. Licensee MDPI, Basel, Switzerland.
PY - 2021/7/6
Y1 - 2021/7/6
N2 - Several ternary composites that are based on branched polyethyleneimine (bPEI 25 kDa, polydispersity 2.5, 0.1 or 0.2 ng), citrate-coated ultrasmall superparamagnetic iron oxide nanoparticles (citrate-NPs, 8-10 nm, 0.1, 1.0, or 2.5 µg), and reporter circular plasmid DNA pEGFP-C1 or pRL-CMV (pDNA 0.5 µg) were studied for optimization of the best composite for transfection into glioblastoma U87MG or U138MG cells. The efficiency in terms of citrate-NP and plasmid DNA gene delivery with the ternary composites could be altered by tuning the bPEI/citrate-NP ratios in the polymer composites, which were characterized by Prussian blue staining, in vitro magnetic resonance imaging as well as green fluorescence protein and luciferase expression. Among the composites prepared, 0.2 ng bPEI/0.5 μg pDNA/1.0 µg citrate-NP ternary composite possessed the best cellular uptake efficiency. Composite comprising 0.1 ng bPEI/0.5 μg pDNA/0.1 μg citrate-NP gave the optimal efficiency for the cellular uptake of the two plasmid DNAs to the nucleus. The best working bPEI concentration range should not exceed 0.2 ng/well to achieve a relatively low cytotoxicity.
AB - Several ternary composites that are based on branched polyethyleneimine (bPEI 25 kDa, polydispersity 2.5, 0.1 or 0.2 ng), citrate-coated ultrasmall superparamagnetic iron oxide nanoparticles (citrate-NPs, 8-10 nm, 0.1, 1.0, or 2.5 µg), and reporter circular plasmid DNA pEGFP-C1 or pRL-CMV (pDNA 0.5 µg) were studied for optimization of the best composite for transfection into glioblastoma U87MG or U138MG cells. The efficiency in terms of citrate-NP and plasmid DNA gene delivery with the ternary composites could be altered by tuning the bPEI/citrate-NP ratios in the polymer composites, which were characterized by Prussian blue staining, in vitro magnetic resonance imaging as well as green fluorescence protein and luciferase expression. Among the composites prepared, 0.2 ng bPEI/0.5 μg pDNA/1.0 µg citrate-NP ternary composite possessed the best cellular uptake efficiency. Composite comprising 0.1 ng bPEI/0.5 μg pDNA/0.1 μg citrate-NP gave the optimal efficiency for the cellular uptake of the two plasmid DNAs to the nucleus. The best working bPEI concentration range should not exceed 0.2 ng/well to achieve a relatively low cytotoxicity.
KW - Citrate
KW - Gene delivery
KW - Magnetic nanoparticle
KW - Nanostructure
KW - Polyethyleneimine
UR - http://www.scopus.com/inward/record.url?scp=85111066963&partnerID=8YFLogxK
U2 - 10.3390/polym13142228
DO - 10.3390/polym13142228
M3 - Journal article
C2 - 34300986
AN - SCOPUS:85111066963
SN - 2073-4360
VL - 13
JO - Polymers
JF - Polymers
IS - 14
M1 - 2228
ER -