TY - JOUR
T1 - Circularly polarised luminescence laser scanning confocal microscopy to study live cell chiral molecular interactions
AU - Stachelek, Patrycja
AU - MacKenzie, Lewis
AU - Parker, David
AU - Pal, Robert
N1 - Funding Information:
R.P. acknowledges support from the Royal Society University Research Fellowship URF\R\191002 and H2020-MSCA-ITN-859752 HEL4CHIROLED. R.P. and P.S. acknowledge support from BBSRC BB/S017615/1, L.M. acknowledges support from the BBSRC Discovery Fellowship BB/T009268/1. P.S. would like to thank Dr. Piotr Pander for his assistance with spin-coating. R.P. thanks Prof. Andrew Beeby for the stimulating discussions regarding CPL spectroscopy. D.P. thanks Dr. Matthieu Starck and Dr. Andrew Frawley for the synthesis of Eu complexes used herein. We are also thankful for the ongoing support of Leica Microsystems UK (Dr. Malcolm Lang, Brian Preston and Jason Lewis) and Coherent UK (Graham Wright and Blair Welsh).
Publisher copyright:
© The Author(s) 2022
PY - 2022/1/27
Y1 - 2022/1/27
N2 - The molecular machinery of life is founded on chiral building blocks, but no experimental technique is currently available to distinguish or monitor chiral systems in live cell bio-imaging studies. Luminescent chiral molecules encode a unique optical fingerprint within emitted circularly polarized light (CPL) carrying information about the molecular environment, conformation, and binding state. Here, we present a CPL Laser Scanning Confocal Microscope (CPL-LSCM) capable of simultaneous chiroptical contrast based live-cell imaging of endogenous and engineered CPL-active cellular probes. Further, we demonstrate that CPL-active probes can be activated using two-photon excitation, with complete CPL spectrum recovery. The combination of these two milestone results empowers the multidisciplinary imaging community, allowing the study of chiral interactions on a sub-cellular level in a new (chiral) light.
AB - The molecular machinery of life is founded on chiral building blocks, but no experimental technique is currently available to distinguish or monitor chiral systems in live cell bio-imaging studies. Luminescent chiral molecules encode a unique optical fingerprint within emitted circularly polarized light (CPL) carrying information about the molecular environment, conformation, and binding state. Here, we present a CPL Laser Scanning Confocal Microscope (CPL-LSCM) capable of simultaneous chiroptical contrast based live-cell imaging of endogenous and engineered CPL-active cellular probes. Further, we demonstrate that CPL-active probes can be activated using two-photon excitation, with complete CPL spectrum recovery. The combination of these two milestone results empowers the multidisciplinary imaging community, allowing the study of chiral interactions on a sub-cellular level in a new (chiral) light.
U2 - 10.1038/s41467-022-28220-z
DO - 10.1038/s41467-022-28220-z
M3 - Journal article
SN - 2041-1723
VL - 13
JO - Nature Communications
JF - Nature Communications
M1 - 553
ER -