TY - JOUR
T1 - Chromosome-level genome assembly of the humpback puffer, Tetraodon palembangensis
AU - Zhang, Rui
AU - Li, Chang
AU - Yu, Mengjun
AU - Huang, Xiaoyun
AU - Zhang, Mengqi
AU - Liu, Shanshan
AU - Pan, Shanshan
AU - Xue, Weizhen
AU - Wang, Congyan
AU - Mao, Chunyan
AU - Zhang, He
AU - Fan, Guangyi
N1 - This work was supported by the special funding of “Blue granary” scientific and technological innovation of China (2018YFD0900301-05).
Publisher Copyright:
© 2020 Phuket Marine Biological Center. All rights reserved.
PY - 2021/4/1
Y1 - 2021/4/1
N2 - The humpback puffer, Tetraodon palembangensis, is a poisonous freshwater pufferfish species mainly distributed in Southeast Asia (Thailand, Laos, Malaysia and Indonesia). The humpback puffer has many interesting biological features, such as inactivity, tetrodotoxin production and body expansion. Here, we report the first chromosome-level genome assembly of the humpback puffer. The genome size is 362 Mb, with a contig N50 value of ~1.78 Mb and a scaffold N50 value of ~15.8 Mb. Based on this genome assembly, ~61.5 Mb (18.11%) repeat sequences were identified, 19,925 genes were annotated, and the function of 90.01% of these genes could be predicted. Finally, a phylogenetic tree of ten teleost fish species was constructed. This analysis suggests that the humpback puffer and T. nigroviridis share a common ancestor 18.1 million years ago (MYA), and diverged from T. rubripes 45.8 MYA. The humpback puffer genome will be a valuable genomic resource to illustrate possible mechanisms of tetrodotoxin synthesis and tolerance.
AB - The humpback puffer, Tetraodon palembangensis, is a poisonous freshwater pufferfish species mainly distributed in Southeast Asia (Thailand, Laos, Malaysia and Indonesia). The humpback puffer has many interesting biological features, such as inactivity, tetrodotoxin production and body expansion. Here, we report the first chromosome-level genome assembly of the humpback puffer. The genome size is 362 Mb, with a contig N50 value of ~1.78 Mb and a scaffold N50 value of ~15.8 Mb. Based on this genome assembly, ~61.5 Mb (18.11%) repeat sequences were identified, 19,925 genes were annotated, and the function of 90.01% of these genes could be predicted. Finally, a phylogenetic tree of ten teleost fish species was constructed. This analysis suggests that the humpback puffer and T. nigroviridis share a common ancestor 18.1 million years ago (MYA), and diverged from T. rubripes 45.8 MYA. The humpback puffer genome will be a valuable genomic resource to illustrate possible mechanisms of tetrodotoxin synthesis and tolerance.
KW - Animal and Plant Sciences
KW - Animal Genetics
KW - Functional Genomics
KW - Marine Biology
UR - http://www.scopus.com/inward/record.url?scp=85181236293&partnerID=8YFLogxK
U2 - 10.46471/gigabyte.17
DO - 10.46471/gigabyte.17
M3 - Journal article
AN - SCOPUS:85181236293
SN - 2709-4715
VL - 2021
JO - GigaByte
JF - GigaByte
ER -