Chromosome 13q12 region critical for the viability and growth of nasopharyngeal carcinoma hybrids

Yue Cheng, Hong Lok Lung, Po Shan Wong, Da Cheng Hao, Chim Shek Man, Eric John Stanbridge, Maria Li Lungi*

*Corresponding author for this work

Research output: Contribution to journalJournal articlepeer-review

12 Citations (Scopus)

Abstract

Allelic losses of chromosome 13 are often detected in nasopharyngeal carcinoma (NPC) and other cancers, implicating the presence of possible tumor suppressor genes (TSGs) on this chromosome. To identify candidate regions from larger and multiple lost areas observed from direct tumor studies, the technique of monochromosome transfer was utilized to provide functional evidence to verify and define these deletion findings. An intact chromosome 13 was transferred into the NPC HONE1 cell line. Resultant hybrids were used to map putative TSG activity. A critical region at 13q12 was non-randomly eliminated in all surviving microcell hybrids around the marker D13S893; these hybrids were uniformly tumorigenic. Although a known TSG, BRCA2, is mapped close to this critical region, no aberrant expression of this gene was detected in microcell hybrids and other NPC cell lines. These results suggest that at least one novel growth control gene on chromosome 13q12, which is not the BRCA2 gene, is essential for hybrid selection and may play a critical role in tumorigenicity.

Original languageEnglish
Pages (from-to)357-362
Number of pages6
JournalInternational Journal of Cancer
Volume109
Issue number3
DOIs
Publication statusPublished - 10 Apr 2004

Scopus Subject Areas

  • Oncology
  • Cancer Research

User-Defined Keywords

  • Chromosome 13
  • Growth suppressor gene
  • Mapping
  • Microcell hybrid
  • Nasopharyngeal carcinoma

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