Chip-based CE coupled to a quadrupole TOF mass spectrometer for the analysis of a glycopeptide

Yufang Zheng, Haifang Li, Zhihong Guo, Jin Ming Lin, Zongwei CAI*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

17 Citations (Scopus)

Abstract

A novel approach using sheath flow chip-based CE coupled to a quadrupole TOF mass spectrometer was developed and applied to the analysis of an O-glycopeptide. The method provided good separation and sensitive analysis of the glycopeptide and its β-elimination product. The β-elimination reaction of the glycopeptide with ammonia or dimethylamine was adapted for changing the glycopeptide into deglycosylated form and for specifying the site of glycosylation. MS/MS analysis of the native peptide, glycopeptide and β-elimination product enabled the identification of the glycosylation site through the comparison of the fragmentations and peptide sequence analysis.

Original languageEnglish
Pages (from-to)1305-1311
Number of pages7
JournalElectrophoresis
Volume28
Issue number9
DOIs
Publication statusPublished - May 2007

Scopus Subject Areas

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry

User-Defined Keywords

  • β-elimination reaction
  • Chip-MS
  • Glycopeptide
  • MS/MS

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