TY - JOUR
T1 - Chemical Profile Assessment and Potential Bioactive Component Screening of a Chinese Patent Herbal Drug Yi-Shen-Hua-Shi Granule
AU - Chan, Yuen-Cheung
AU - Jing, Zhao
AU - Qin, Hu
AU - Hui, Guo
AU - Liu, Yu-Xi
AU - Wu, Jia-Ying
AU - Zheng, Zhao-Guang
AU - Yu, Zhi-Ling
N1 - Funding Information:
The author(s) disclosed receipt of the following financial support for the research, authorship,and/or publication of this article: This research was supported by Science and Technology Key Project of Covid-19 in Foshan City (grant No.: 2020001000206) and the Innovation and Technology Commission of Hong Kong (grant No.: UIM/290).
Publisher Copyright:
© The Author(s) 2021.
PY - 2021/6
Y1 - 2021/6
N2 - Yi-Shen-Hua-Shi (YSHS) Granule is a well-known patented herbal drug for treating chronic glomerulonephritis (CGN) in China. Its chemical compositions and anti-CGN components are not fully understood. This study aimed to establish the chemical profile and to identify the anti-CGN components of YSHS Granule. For these purposes, a HPLC-Q-TOF-MS/MS method was developed. A total of 105 peaks were detected in the mass spectrum of the Granule. Of these, 99 compounds were tentatively identified as terpenoids, flavonoids, coumarins, alkaloids, phenols, and other types of compounds, and 15 were further identified with reference substances. To screen bioactive compounds, a cell membrane immobilized chromatography (CMIC) method was used. Lipopolysaccharide (LPS)-challenged rat glomerular mesangial cells (HBZY-1) were incubated with YSHS Granule (100 μg/mL), and the binding components to the cell membrane were extracted and analyzed using the established HPLC-Q-TOF-MS/MS method. Seven potential bioactive components that bound to HBZY-1 cell membranes were detected and identified as calycosin-7-O-β-D-glucoside, 6-gingerol, naringin, ginsenoside Re, poncirin, liquiritigenin, and isoliquiritigenin. The outcomes of the present study provide a chemical basis for clinical use of the Granule in managing CGN, and provide quality control markers for the Granule’s production and use.
AB - Yi-Shen-Hua-Shi (YSHS) Granule is a well-known patented herbal drug for treating chronic glomerulonephritis (CGN) in China. Its chemical compositions and anti-CGN components are not fully understood. This study aimed to establish the chemical profile and to identify the anti-CGN components of YSHS Granule. For these purposes, a HPLC-Q-TOF-MS/MS method was developed. A total of 105 peaks were detected in the mass spectrum of the Granule. Of these, 99 compounds were tentatively identified as terpenoids, flavonoids, coumarins, alkaloids, phenols, and other types of compounds, and 15 were further identified with reference substances. To screen bioactive compounds, a cell membrane immobilized chromatography (CMIC) method was used. Lipopolysaccharide (LPS)-challenged rat glomerular mesangial cells (HBZY-1) were incubated with YSHS Granule (100 μg/mL), and the binding components to the cell membrane were extracted and analyzed using the established HPLC-Q-TOF-MS/MS method. Seven potential bioactive components that bound to HBZY-1 cell membranes were detected and identified as calycosin-7-O-β-D-glucoside, 6-gingerol, naringin, ginsenoside Re, poncirin, liquiritigenin, and isoliquiritigenin. The outcomes of the present study provide a chemical basis for clinical use of the Granule in managing CGN, and provide quality control markers for the Granule’s production and use.
KW - chronic glomerulonephritis
KW - chemical profile
KW - cell membrane immobilized chromatography
KW - HPLC-Q-TOF-MS/MS
KW - bioactive components
KW - Yi-Shen-Hua-Shi Granule
UR - http://www.scopus.com/inward/record.url?scp=85107739939&partnerID=8YFLogxK
U2 - 10.1177/1934578X211021691
DO - 10.1177/1934578X211021691
M3 - Journal article
AN - SCOPUS:85107739939
SN - 1934-578X
VL - 16
SP - 1
EP - 17
JO - Natural Product Communications
JF - Natural Product Communications
IS - 6
ER -