Characterization of two near-infrared genetically encoded voltage indicators

Chenchen Song, Mikhail E. Matlashov, Daria M. Shcherbakova, Srdjan D. Antic, Srdjan D. Antic, Vladislav V. Verkhusha, Thomas Knöpfel*

*Corresponding author for this work

Research output: Contribution to journalJournal articlepeer-review


Significance: Efforts starting more than 20 years ago led to increasingly well performing genetically encoded voltage indicators (GEVIs) for optical imaging at wavelengths <600 nm. Although optical imaging in the >600 nm wavelength range has many advantages over shorter wavelength approaches for mesoscopic in vivo monitoring of neuronal activity in the mammalian brain, the availability and evaluation of well performing near-infrared GEVIs are still limited.

Aim: Here, we characterized two recent near-infrared GEVIs, Archon1 and nirButterfly, to support interested tool users in selecting a suitable near-infrared GEVI for their specific research question requirements.

Approach: We characterized side-by-side the brightness, sensitivity, and kinetics of both near-infrared GEVIs in a setting focused on population imaging.

Results: We found that nirButterfly shows seven-fold higher brightness than Archon1 under the same conditions and faster kinetics than Archon1 for population imaging without cellular resolution. But Archon1 showed larger signals than nirButterfly.

Conclusions: Neither GEVI characterized here surpasses in all three key parameters (brightness, kinetics, and sensitivity), so there is no unequivocal preference for one of the two. Our side-by-side characterization presented here provides new information for future in vitro and ex vivo experimental designs.
Original languageEnglish
Article number024201
Number of pages10
Issue number2
Early online date11 Dec 2023
Publication statusE-pub ahead of print - 11 Dec 2023

User-Defined Keywords

  • genetically encoded voltage indicator
  • voltage imaging
  • wide-field optical imaging
  • opsin
  • voltage-sensing domain
  • iRFP


Dive into the research topics of 'Characterization of two near-infrared genetically encoded voltage indicators'. Together they form a unique fingerprint.

Cite this