Characterization of cues from natural multi-species biofilms that induce larval attachment of the polychaete Hydroides elegans

Oi Shing Hung, On On Lee, Vengatesen Thiyagarajan, Hong Ping He, Ying Xu, Hong Chun Chung, Jianwen QIU, Pei Yuan Qian*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

23 Citations (Scopus)

Abstract

Multi-species natural microbial biofilms have been identified as sources of cues to induce larval attachment of a broad range of sessile marine invertebrates. However, the chemical identities of the cues originating from these films have not been fully characterized. In this study, we isolated and characterized 2 compounds from natural biofilms that induced the larval attachment of the polychaete Hydroides elegans. Biofilms were developed on Petri dishes in seawater in the field for 6 d, collected from the Petri dishes using cotton buds, and extracted using a combination of polar and non-polar solvents. The non-polar fraction was inductive to larval attachment and was thus further fractionated using HPLC. Bioassay-guided fractionation was used to locate the active compounds that were then structurally characterized by using nuclear magnetic resonance (NMR) spectroscopy. Two inductive compounds were identified, a hydrocarbon (6,9-heptadecadiene) and a fatty acid (12-octadecenoic acid), which are the first chemical cues inductive to larval attachment of marine invertebrates isolated from natural biofilms.

Original languageEnglish
Pages (from-to)253-262
Number of pages10
JournalAquatic Biology
Volume4
Issue number3
DOIs
Publication statusPublished - 2008

Scopus Subject Areas

  • Oceanography
  • Ecology, Evolution, Behavior and Systematics
  • Aquatic Science
  • Ecology

User-Defined Keywords

  • Fatty acid
  • Hydrocarbon
  • Hydroides elegans
  • Inductive cues
  • Natural biofilms

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