Characterization of a regulatory region in the N-terminus of rabbit Kv1.3

Xiaoqiang Yao*, Yu Huang, Hiu Yee Kwan, Pat Chan, Gary Desir

*Corresponding author for this work

Research output: Contribution to journalJournal articlepeer-review

5 Citations (Scopus)


The N-terminus of rabbit Kv1.3 contains a signal sequence which regulates expression of Kv1.3 proteins in the plasma membrane. Removal of an N-terminal region (aa3-39) produced an increase in expressed K+ current. Progressive deletion at the N-terminus demonstrated that the shortest deletion required for the elevation of K current is D6-34. Since the functional signal sequence must include both ends of the peptide segment aa6-34 where charged residues are densely distributed, it is conceivable that this N-terminal signal sequence is related to charge or its associated hydrophilicity. Removal of two charged residues (31R, 33E) through amino acid substitution which converts deletion construct D3-27 to D3-27* effectively raises the amplitude of expressed current, further indicating the importance of charged residues. With the use of a mutated dynamin and a soluble N-terminal peptide, we also revealed that the N-terminal signal sequence may not act through the endocytotic pathway.

Original languageEnglish
Pages (from-to)492-498
Number of pages7
JournalBiochemical and Biophysical Research Communications
Issue number2
Publication statusPublished - 19 Aug 1998

Scopus Subject Areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology


Dive into the research topics of 'Characterization of a regulatory region in the N-terminus of rabbit Kv1.3'. Together they form a unique fingerprint.

Cite this