TY - JOUR
T1 - Characterization and quantification of flavonoids and saponins in adzuki bean (Vigna angularis L.) by HPLC-DAD-ESI-MSn analysis
AU - Liu, Rui
AU - Cai, Zongwei
AU - Xu, Baojun
N1 - Funding Information:
This project is jointly supported UIC internal grant (Project Codes: R201627 and R201714) from Beijing Normal University-Hong Kong Baptist University United International College, China.
PY - 2017/9/22
Y1 - 2017/9/22
N2 - Background: Bioactive activities of adzuki bean have been widely reported, however, the phytochemical information of adzuki bean is incomplete. The aim of this study was to characterize and quantify flavonoids and saponins in adzuki bean. High performance liquid chromatography with diode array detection and electro spray ionization-tandem multi-stage mass spectrometry (HPLC-DAD-ESI-MSn) were applied to do qualitative and quantitative analyses. Results: A total of 15 compounds from adzuki bean were identified by HPLC-DAD-ESI-MSn. Among 15 compounds identified, four flavonoids (catechin, vitexin-4"-O-glucoside, quercetin-3-O-glucoside, and quercetin-3-O-rutinoside) and six saponins (azukisaponin I, II, III, IV, V, and VI) in adzuki bean were further quantified by external calibration method using HPLC-MS with the program of time segment and extract ion chromatogram (EIC) analysis. Conclusions: Current qualitative and quantitative method based on HPLC and MS technique provides a scientific basis for in vitro and in vivo pharmacological study in the future.[Figure not available: see fulltext.]
AB - Background: Bioactive activities of adzuki bean have been widely reported, however, the phytochemical information of adzuki bean is incomplete. The aim of this study was to characterize and quantify flavonoids and saponins in adzuki bean. High performance liquid chromatography with diode array detection and electro spray ionization-tandem multi-stage mass spectrometry (HPLC-DAD-ESI-MSn) were applied to do qualitative and quantitative analyses. Results: A total of 15 compounds from adzuki bean were identified by HPLC-DAD-ESI-MSn. Among 15 compounds identified, four flavonoids (catechin, vitexin-4"-O-glucoside, quercetin-3-O-glucoside, and quercetin-3-O-rutinoside) and six saponins (azukisaponin I, II, III, IV, V, and VI) in adzuki bean were further quantified by external calibration method using HPLC-MS with the program of time segment and extract ion chromatogram (EIC) analysis. Conclusions: Current qualitative and quantitative method based on HPLC and MS technique provides a scientific basis for in vitro and in vivo pharmacological study in the future.[Figure not available: see fulltext.]
KW - Adzuki bean
KW - ESI-MS
KW - Flavonoids
KW - HPLC
KW - Saponins
UR - http://www.scopus.com/inward/record.url?scp=85029888164&partnerID=8YFLogxK
U2 - 10.1186/s13065-017-0317-x
DO - 10.1186/s13065-017-0317-x
M3 - Journal article
AN - SCOPUS:85029888164
SN - 1752-153X
VL - 11
JO - Chemistry Central Journal
JF - Chemistry Central Journal
IS - 1
M1 - 93
ER -