TY - JOUR
T1 - Characterization and determination of six flavonoids in the ethnomedicine "Dragon's Blood" by UPLC-PAD-MS
AU - Yi, Tao
AU - Tang, Yina
AU - Zhang, Jianye
AU - Zhao, Zhongzhen
AU - Yang, Zhijun
AU - Chen, Hubiao
N1 - Copyright:
Copyright 2012 Elsevier B.V., All rights reserved.
PY - 2012/10/10
Y1 - 2012/10/10
N2 - Background: " Dragon's Blood" (DB) has long been used as an ethnomedicine in China to invigorate blood circulation for the treatment of traumatic injuries, blood stasis and pain. To comprehensively assess the quality of DB medicine, a precise and accurate method that can rapidly separate, characterize and quantify multiple active components of DB is crucial.Results: An ultra performance liquid chromatography (UPLC) coupled with photodiode array detection (PAD) and electrospray ionization mass spectrometry (ESI-MS) method was developed for characterization and determination of six flavonoids in DB. A comprehensive validation of the developed method was conducted, and confirmed that the method presented good sensitivity, precision and accuracy. All linear regressions were acquired with R 2 > 0.99, and the limits of detection ranged from 0.06 to 0.83 ng. The relative standard deviation (RSD) values were found to be within the range 1.4-3.8% for the method repeatability test. Recovery studies for the quantified compounds were found to be within the range 94.2-102.8% with RSD less than 4.9%. DB samples collected from different geographical regions were analyzed by the present method, and the results demonstrated that the contents of the six flavonoids in DB samples varied significantly. Three major active components among the six flavonoids, namely dracorhodin, (2S)-5-methoxyflavan-7-ol and (2S)-5-methoxy-6-methylflavan-7-ol, are suggested as the index for DB quality evaluation.Conclusions: Overall, the present hyphenation method is highly efficient and reliable, and hence suitable for the characterization and determination of the flavonoids of DB ethnomedicine.
AB - Background: " Dragon's Blood" (DB) has long been used as an ethnomedicine in China to invigorate blood circulation for the treatment of traumatic injuries, blood stasis and pain. To comprehensively assess the quality of DB medicine, a precise and accurate method that can rapidly separate, characterize and quantify multiple active components of DB is crucial.Results: An ultra performance liquid chromatography (UPLC) coupled with photodiode array detection (PAD) and electrospray ionization mass spectrometry (ESI-MS) method was developed for characterization and determination of six flavonoids in DB. A comprehensive validation of the developed method was conducted, and confirmed that the method presented good sensitivity, precision and accuracy. All linear regressions were acquired with R 2 > 0.99, and the limits of detection ranged from 0.06 to 0.83 ng. The relative standard deviation (RSD) values were found to be within the range 1.4-3.8% for the method repeatability test. Recovery studies for the quantified compounds were found to be within the range 94.2-102.8% with RSD less than 4.9%. DB samples collected from different geographical regions were analyzed by the present method, and the results demonstrated that the contents of the six flavonoids in DB samples varied significantly. Three major active components among the six flavonoids, namely dracorhodin, (2S)-5-methoxyflavan-7-ol and (2S)-5-methoxy-6-methylflavan-7-ol, are suggested as the index for DB quality evaluation.Conclusions: Overall, the present hyphenation method is highly efficient and reliable, and hence suitable for the characterization and determination of the flavonoids of DB ethnomedicine.
KW - Characterization
KW - Daemonorops draco
KW - Dragon's Blood
KW - Quality evaluation
KW - UPLC-PAD-MS
UR - http://www.scopus.com/inward/record.url?scp=84867210407&partnerID=8YFLogxK
U2 - 10.1186/1752-153X-6-116
DO - 10.1186/1752-153X-6-116
M3 - Journal article
AN - SCOPUS:84867210407
SN - 1752-153X
VL - 6
JO - Chemistry Central Journal
JF - Chemistry Central Journal
IS - 1
M1 - 116
ER -