Chapter Seven Biopanning by activation tagging

Yiji Xia*, Justin Borevitz, Jack W. Blount, Richard A. Dixon, Chris Lamb

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

This chapter focuses on biopanning by activation tagging. Extensive gene duplication in plants and the potential of functional redundancy among homologous sequences represent a hurdle in understanding physiological and biochemical functions of a vast number of plant genes by using conventional loss-of-function mutant screen approaches. Gain-of-function mutations created by activation tagging might provide important clues about functions of the activated genes. The identification of PAP1 demonstrates the usefulness of activation tagging for generating massively enriched tissue and plant sources for production of specific natural products and isolating key genes regulating their biosynthetic pathways. Activation tagging has successfully been applied to Arabidopsis as well as to other plant species for cloning genes affecting various biological processes and represents a powerful approach for functional genomics.

Original languageEnglish
Pages (from-to)111-123
Number of pages13
JournalRecent Advances in Phytochemistry
Volume36
DOIs
Publication statusPublished - Jan 2002
Externally publishedYes

Scopus Subject Areas

  • Biochemistry
  • Molecular Biology
  • Plant Science
  • Cell Biology

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