Changes of ATP and ADP in cultured astrocytes under and after in vitro ischemia

Albert Cheung Hoi Yu; Anson Ming Fung Lau; Ada Wing Yu Fu; Lok Ting Lau; Philip Yeung Lam; Xiao Qian Chen; Zhen Yu Xu

doi:10.1023/A:1021691112190

Changes of ATP and ADP in cultured astrocytes under and after in vitro ischemia

Albert Cheung Hoi Yu^*, Anson Ming Fung Lau, Ada Wing Yu Fu, Lok Ting Lau, Philip Yeung Lam, Xiao Qian Chen, Zhen Yu Xu

^*Corresponding author for this work

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Abstract

A very large body of evidence from in vivo studies has been accumulated on a link between the change of energy and cell survival/apoptosis. Using an in vitro ischemia model, we have previously shown that ischemia could induce apoptosis in astrocytes. In this study, we utilized the same in vitro model to investigate changes in ATP and ADP levels in cultured astrocytes and attempted to demonstrate an energy - cell death linkage. Astrocytes remained unaltered after 2 hr of ischemia but were moderately or severely damaged after 4 or 6-8 hr, respectively. The astrocytes that survived various lengths of in vitro ischemic incubation retained their ability to produce ATP after ischemia. Both ATP and ADP levels were increased in astrocytes that remained alive under in vitro ischemia for over 6 hr. The largest decline in the percent of viable astrocytes during ischemia corresponded well to the reduction in ATP and ADP levels in these cultures.

Original language	English
Pages (from-to)	1663-1668
Number of pages	6
Journal	Neurochemical Research
Volume	27
DOIs	https://doi.org/10.1023/A:1021691112190
Publication status	Published - 1 Dec 2002

Scopus Subject Areas

Biochemistry
Cellular and Molecular Neuroscience

User-Defined Keywords

Astrocytes
ATP
Culture
Energy metabolism
In vitro ischemia

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10.1023/A:1021691112190

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title = "Changes of ATP and ADP in cultured astrocytes under and after in vitro ischemia",

abstract = "A very large body of evidence from in vivo studies has been accumulated on a link between the change of energy and cell survival/apoptosis. Using an in vitro ischemia model, we have previously shown that ischemia could induce apoptosis in astrocytes. In this study, we utilized the same in vitro model to investigate changes in ATP and ADP levels in cultured astrocytes and attempted to demonstrate an energy - cell death linkage. Astrocytes remained unaltered after 2 hr of ischemia but were moderately or severely damaged after 4 or 6-8 hr, respectively. The astrocytes that survived various lengths of in vitro ischemic incubation retained their ability to produce ATP after ischemia. Both ATP and ADP levels were increased in astrocytes that remained alive under in vitro ischemia for over 6 hr. The largest decline in the percent of viable astrocytes during ischemia corresponded well to the reduction in ATP and ADP levels in these cultures.",

keywords = "Astrocytes, ATP, Culture, Energy metabolism, In vitro ischemia",

author = "Yu, {Albert Cheung Hoi} and Lau, {Anson Ming Fung} and Fu, {Ada Wing Yu} and Lau, {Lok Ting} and Lam, {Philip Yeung} and Chen, {Xiao Qian} and Xu, {Zhen Yu}",

note = "Funding Information: We thank Dr. Richard Collins and Elaine Young for their assistance in the preparation of this manuscript. This work was sup- ported by grants from the Research Grant Council (H.K.) DAG 95/96.SC07, HKUST6177/97M, the North American Medical Association Foundation (Hong Kong) NAMA 94/95.SC01, and British Council Grant JRS96/34 to ACHY. W.Y.F. and L.T.L. were graduate students; A.M.F.L. and P.Y.L. were undergraduate students from the Department of Biology, HKUST. Publisher copyright: {\textcopyright} 2002 Plenum Publishing Corporation",

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AU - Yu, Albert Cheung Hoi

AU - Lau, Anson Ming Fung

AU - Fu, Ada Wing Yu

AU - Lau, Lok Ting

AU - Lam, Philip Yeung

AU - Chen, Xiao Qian

AU - Xu, Zhen Yu

N1 - Funding Information: We thank Dr. Richard Collins and Elaine Young for their assistance in the preparation of this manuscript. This work was sup- ported by grants from the Research Grant Council (H.K.) DAG 95/96.SC07, HKUST6177/97M, the North American Medical Association Foundation (Hong Kong) NAMA 94/95.SC01, and British Council Grant JRS96/34 to ACHY. W.Y.F. and L.T.L. were graduate students; A.M.F.L. and P.Y.L. were undergraduate students from the Department of Biology, HKUST. Publisher copyright: © 2002 Plenum Publishing Corporation

PY - 2002/12/1

Y1 - 2002/12/1

N2 - A very large body of evidence from in vivo studies has been accumulated on a link between the change of energy and cell survival/apoptosis. Using an in vitro ischemia model, we have previously shown that ischemia could induce apoptosis in astrocytes. In this study, we utilized the same in vitro model to investigate changes in ATP and ADP levels in cultured astrocytes and attempted to demonstrate an energy - cell death linkage. Astrocytes remained unaltered after 2 hr of ischemia but were moderately or severely damaged after 4 or 6-8 hr, respectively. The astrocytes that survived various lengths of in vitro ischemic incubation retained their ability to produce ATP after ischemia. Both ATP and ADP levels were increased in astrocytes that remained alive under in vitro ischemia for over 6 hr. The largest decline in the percent of viable astrocytes during ischemia corresponded well to the reduction in ATP and ADP levels in these cultures.

AB - A very large body of evidence from in vivo studies has been accumulated on a link between the change of energy and cell survival/apoptosis. Using an in vitro ischemia model, we have previously shown that ischemia could induce apoptosis in astrocytes. In this study, we utilized the same in vitro model to investigate changes in ATP and ADP levels in cultured astrocytes and attempted to demonstrate an energy - cell death linkage. Astrocytes remained unaltered after 2 hr of ischemia but were moderately or severely damaged after 4 or 6-8 hr, respectively. The astrocytes that survived various lengths of in vitro ischemic incubation retained their ability to produce ATP after ischemia. Both ATP and ADP levels were increased in astrocytes that remained alive under in vitro ischemia for over 6 hr. The largest decline in the percent of viable astrocytes during ischemia corresponded well to the reduction in ATP and ADP levels in these cultures.

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KW - Culture

KW - Energy metabolism

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DO - 10.1023/A:1021691112190

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JO - Neurochemical Research

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ER -

Changes of ATP and ADP in cultured astrocytes under and after in vitro ischemia

Abstract

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