cGMP stimulates endoplasmic reticulum Ca2+-ATPase in vascular endothelial cells

Kin Ling Lau, Siu Kai Kong, Wing Hung Ko, Hiu Yee Kwan, Yu Huang, Xiaoqiang Yao*

*Corresponding author for this work

Research output: Contribution to journalJournal articlepeer-review

18 Citations (Scopus)


Calcium is a crucial regulator of many physiological processes such as cell growth, division, differentiation, cell death and apoptosis. In this study, we examined the effect of cGMP on agonist-induced [Ca2+]i transient in isolated rat aortic endothelial cells. 100 μM ATP was applied to the cells bathed in a Ca2+-free physiological solution to induce a [Ca2+]i transient that was caused by Ca2+ release from intracellular stores. cGMP, which was applied after [Ca2+]i reached its peak level, accelerated the falling phase of [Ca2+]i transient. Pre-treatment of the cells with CPA abolished the accelerating effect of cGMP on the falling phase of [Ca2+]i transient. The effect of cGMP was reversed by KT5823, a highly specific inhibitor of protein kinase G. Taken together, these data suggest that cGMP may reduce [Ca2+]i level by promoting Ca2+ uptake through sarcoplasmic/endoplasmic reticulum ATPase and that the effect of cGMP may be mediated by protein kinase G.

Original languageEnglish
Pages (from-to)2019-2028
Number of pages10
JournalLife Sciences
Issue number16
Publication statusPublished - 5 Sept 2003

Scopus Subject Areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)

User-Defined Keywords

  • cGMP
  • Endothelial cells
  • PKG


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