Cellular uptake, subcellular localization and photodamaging effect of Temoporfin (mTHPC) in nasopharyngeal carcinoma cells: Comparison with hematoporphyrin derivative

C. M.N. Yow; J. Y. Chen; Nai Ki Mak; Nai Ho Cheung; A. W.N. Leung

doi:10.1016/S0304-3835(00)00453-5

Cellular uptake, subcellular localization and photodamaging effect of Temoporfin (mTHPC) in nasopharyngeal carcinoma cells: Comparison with hematoporphyrin derivative

C. M.N. Yow^*, J. Y. Chen, Nai Ki Mak, Nai Ho Cheung, A. W.N. Leung

^*Corresponding author for this work

Research output: Contribution to journal › Journal article › peer-review

84 Citations (Scopus)

Abstract

Temoporfin (meta-tetra (hydroxyphenyl)chlorin; mTHPC) potentiated a 100- fold higher cytotoxic effect than hematoporphyrin derivative (HPD) on two nasopharyngeal carcinoma cell lines (HK1 and CNE2) in terms of the overall photodynamic therapy (PDT) dose. The cellular uptake, evaluated by flow cytometry and spectrophotometry demonstrated that mTHPC exhibited higher uptake ability than HPD. Confocal laser scanning microscopy detection for both the sensitizer and mitochondria probe on the same cell images revealed that both drugs accumulated diffusely in the cytoplasm and that mitochrondria is a target organelle. Photo-activation ruptured the mitochrondria, with more pronounced mitochondrial damage being observed in mTHPC-PDT course. This correlated well with the cell photokilling efficiency of mTHPC. (C) 2000 Elsevier Science Ireland Ltd.

Original language	English
Article number	6035
Pages (from-to)	123-131
Number of pages	9
Journal	Cancer Letters
Volume	157
Issue number	2
DOIs	https://doi.org/10.1016/S0304-3835(00)00453-5
Publication status	Published - 1 Sept 2000

Scopus Subject Areas

Oncology
Cancer Research

User-Defined Keywords

Nasopharyngeal carcinoma
Photodynamic therapy
Photosensitizers

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/S0304-3835(00)00453-5

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title = "Cellular uptake, subcellular localization and photodamaging effect of Temoporfin (mTHPC) in nasopharyngeal carcinoma cells: Comparison with hematoporphyrin derivative",

abstract = "Temoporfin (meta-tetra (hydroxyphenyl)chlorin; mTHPC) potentiated a 100- fold higher cytotoxic effect than hematoporphyrin derivative (HPD) on two nasopharyngeal carcinoma cell lines (HK1 and CNE2) in terms of the overall photodynamic therapy (PDT) dose. The cellular uptake, evaluated by flow cytometry and spectrophotometry demonstrated that mTHPC exhibited higher uptake ability than HPD. Confocal laser scanning microscopy detection for both the sensitizer and mitochondria probe on the same cell images revealed that both drugs accumulated diffusely in the cytoplasm and that mitochrondria is a target organelle. Photo-activation ruptured the mitochrondria, with more pronounced mitochondrial damage being observed in mTHPC-PDT course. This correlated well with the cell photokilling efficiency of mTHPC. (C) 2000 Elsevier Science Ireland Ltd.",

keywords = "Nasopharyngeal carcinoma, Photodynamic therapy, Photosensitizers",

author = "Yow, {C. M.N.} and Chen, {J. Y.} and Mak, {Nai Ki} and Cheung, {Nai Ho} and Leung, {A. W.N.}",

note = "Funding Information: We thank Scotia Pharmaceuticals Ltd. for their gift of the mTHPC samples, to Dr D.P. Huang for providing the cell lines, to the Earmarked Grant of the University Grant Committee of Hong Kong, the Hong Kong Polytechnic University, the Hong Kong Baptist University and the National Natural Science Foundation of China for their support of this work.",

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AU - Yow, C. M.N.

AU - Chen, J. Y.

AU - Mak, Nai Ki

AU - Cheung, Nai Ho

AU - Leung, A. W.N.

N1 - Funding Information: We thank Scotia Pharmaceuticals Ltd. for their gift of the mTHPC samples, to Dr D.P. Huang for providing the cell lines, to the Earmarked Grant of the University Grant Committee of Hong Kong, the Hong Kong Polytechnic University, the Hong Kong Baptist University and the National Natural Science Foundation of China for their support of this work.

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N2 - Temoporfin (meta-tetra (hydroxyphenyl)chlorin; mTHPC) potentiated a 100- fold higher cytotoxic effect than hematoporphyrin derivative (HPD) on two nasopharyngeal carcinoma cell lines (HK1 and CNE2) in terms of the overall photodynamic therapy (PDT) dose. The cellular uptake, evaluated by flow cytometry and spectrophotometry demonstrated that mTHPC exhibited higher uptake ability than HPD. Confocal laser scanning microscopy detection for both the sensitizer and mitochondria probe on the same cell images revealed that both drugs accumulated diffusely in the cytoplasm and that mitochrondria is a target organelle. Photo-activation ruptured the mitochrondria, with more pronounced mitochondrial damage being observed in mTHPC-PDT course. This correlated well with the cell photokilling efficiency of mTHPC. (C) 2000 Elsevier Science Ireland Ltd.

AB - Temoporfin (meta-tetra (hydroxyphenyl)chlorin; mTHPC) potentiated a 100- fold higher cytotoxic effect than hematoporphyrin derivative (HPD) on two nasopharyngeal carcinoma cell lines (HK1 and CNE2) in terms of the overall photodynamic therapy (PDT) dose. The cellular uptake, evaluated by flow cytometry and spectrophotometry demonstrated that mTHPC exhibited higher uptake ability than HPD. Confocal laser scanning microscopy detection for both the sensitizer and mitochondria probe on the same cell images revealed that both drugs accumulated diffusely in the cytoplasm and that mitochrondria is a target organelle. Photo-activation ruptured the mitochrondria, with more pronounced mitochondrial damage being observed in mTHPC-PDT course. This correlated well with the cell photokilling efficiency of mTHPC. (C) 2000 Elsevier Science Ireland Ltd.

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Cellular uptake, subcellular localization and photodamaging effect of Temoporfin (mTHPC) in nasopharyngeal carcinoma cells: Comparison with hematoporphyrin derivative

Abstract

Scopus Subject Areas

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UN SDGs

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