Cellular uptake, subcellular localization and photodamaging effect of Temoporfin (mTHPC) in nasopharyngeal carcinoma cells: Comparison with hematoporphyrin derivative

C. M.N. Yow*, J. Y. Chen, Nai Ki MAK, Nai Ho CHEUNG, A. W.N. Leung

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

79 Citations (Scopus)

Abstract

Temoporfin (meta-tetra (hydroxyphenyl)chlorin; mTHPC) potentiated a 100- fold higher cytotoxic effect than hematoporphyrin derivative (HPD) on two nasopharyngeal carcinoma cell lines (HK1 and CNE2) in terms of the overall photodynamic therapy (PDT) dose. The cellular uptake, evaluated by flow cytometry and spectrophotometry demonstrated that mTHPC exhibited higher uptake ability than HPD. Confocal laser scanning microscopy detection for both the sensitizer and mitochondria probe on the same cell images revealed that both drugs accumulated diffusely in the cytoplasm and that mitochrondria is a target organelle. Photo-activation ruptured the mitochrondria, with more pronounced mitochondrial damage being observed in mTHPC-PDT course. This correlated well with the cell photokilling efficiency of mTHPC. (C) 2000 Elsevier Science Ireland Ltd.

Original languageEnglish
Article number6035
Pages (from-to)123-131
Number of pages9
JournalCancer Letters
Volume157
Issue number2
DOIs
Publication statusPublished - 2000

Scopus Subject Areas

  • Oncology
  • Cancer Research

User-Defined Keywords

  • Nasopharyngeal carcinoma
  • Photodynamic therapy
  • Photosensitizers

Fingerprint

Dive into the research topics of 'Cellular uptake, subcellular localization and photodamaging effect of Temoporfin (mTHPC) in nasopharyngeal carcinoma cells: Comparison with hematoporphyrin derivative'. Together they form a unique fingerprint.

Cite this