CDK5RAP2 is a Wnt target gene and promotes stemness and progression of oral squamous cell carcinoma

Yuehong Shen; Yuling Chen; Yuntao Lin; Yicun Li; Pengfei Liu; Biru Zhang; Yufan Wang; King Chi Chan; Nai Ki Mak; Michael Kahn; Robert Z. Qi; Hongyu Yang

doi:10.1038/s41419-023-05652-z

CDK5RAP2 is a Wnt target gene and promotes stemness and progression of oral squamous cell carcinoma

Yuehong Shen, Yuling Chen, Yuntao Lin, Yicun Li, Pengfei Liu, Biru Zhang, Yufan Wang, King Chi Chan, Nai Ki Mak, Michael Kahn, Robert Z. Qi^*, Hongyu Yang^*

^*Corresponding author for this work

Department of Biology

Research output: Contribution to journal › Journal article › peer-review

7 Citations (Scopus)

Abstract

In oral squamous cell carcinoma (OSCC), a highly aggressive and frequently lethal malignancy, the role and action mechanism of the microtubule regulatory protein CDK5RAP2 have not been fully understood. Here, we show that CDK5RAP2 is highly expressed in OSCC and its expression correlates with clinical stage and lymph node metastasis of the disease. The expression of CDK5RAP2 is regulated by the Wnt signaling pathway. Depletion of CDK5RAP2 inhibits the tumorigenesis and migration of OSCC cells and alters the OSCC cancer stem (-like) cell (CSC) signature. Notably, suppression of CDK5RAP2 expression disrupts spindle orientation during mitosis. Collectively, these results identify CDK5RAP2 as a potential CSC marker and reveal a mechanism that controls the CSC population in OSCC.

Original language	English
Article number	107
Number of pages	11
Journal	Cell Death and Disease
Volume	14
DOIs	https://doi.org/10.1038/s41419-023-05652-z
Publication status	Published - 11 Feb 2023

Scopus Subject Areas

Immunology
Cellular and Molecular Neuroscience
Cell Biology
Cancer Research

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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title = "CDK5RAP2 is a Wnt target gene and promotes stemness and progression of oral squamous cell carcinoma",

abstract = "In oral squamous cell carcinoma (OSCC), a highly aggressive and frequently lethal malignancy, the role and action mechanism of the microtubule regulatory protein CDK5RAP2 have not been fully understood. Here, we show that CDK5RAP2 is highly expressed in OSCC and its expression correlates with clinical stage and lymph node metastasis of the disease. The expression of CDK5RAP2 is regulated by the Wnt signaling pathway. Depletion of CDK5RAP2 inhibits the tumorigenesis and migration of OSCC cells and alters the OSCC cancer stem (-like) cell (CSC) signature. Notably, suppression of CDK5RAP2 expression disrupts spindle orientation during mitosis. Collectively, these results identify CDK5RAP2 as a potential CSC marker and reveal a mechanism that controls the CSC population in OSCC.",

author = "Yuehong Shen and Yuling Chen and Yuntao Lin and Yicun Li and Pengfei Liu and Biru Zhang and Yufan Wang and Chan, {King Chi} and Mak, {Nai Ki} and Michael Kahn and Qi, {Robert Z.} and Hongyu Yang",

note = "We thank Lu Fu for assistance in conducting ChIP and Luciferase assays. Thank the Bioinformatics Platform, Peking University Shenzhen Hospital for assistance in data analysis. This work was supported by grants from the National Natural Science Foundation of China (81902758), Shenzhen Science and Technology Innovation Committee (JCYJ20190809104803572 and SGDX20210823103200005), Shenzhen Fund for Guangdong Provincial High-level Clinical Key Specialties (No. SZGSP008), Sanming Project of Medicine in Shenzhen (SZSM 202111012, Prof. Guangyan Yu, Oral and Maxillofacial Surgery Team, Peking University Hospital of Stomatology), Research Grants Council (General Research Fund) of Hong Kong, and the Innovation and Technology Commission (ITCPD/17-9) of Hong Kong. Publisher Copyright: {\textcopyright} The Author(s) 2023",

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doi = "10.1038/s41419-023-05652-z",

language = "English",

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journal = "Cell Death and Disease",

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TY - JOUR

T1 - CDK5RAP2 is a Wnt target gene and promotes stemness and progression of oral squamous cell carcinoma

AU - Shen, Yuehong

AU - Chen, Yuling

AU - Lin, Yuntao

AU - Li, Yicun

AU - Liu, Pengfei

AU - Zhang, Biru

AU - Wang, Yufan

AU - Chan, King Chi

AU - Mak, Nai Ki

AU - Kahn, Michael

AU - Qi, Robert Z.

AU - Yang, Hongyu

N1 - We thank Lu Fu for assistance in conducting ChIP and Luciferase assays. Thank the Bioinformatics Platform, Peking University Shenzhen Hospital for assistance in data analysis. This work was supported by grants from the National Natural Science Foundation of China (81902758), Shenzhen Science and Technology Innovation Committee (JCYJ20190809104803572 and SGDX20210823103200005), Shenzhen Fund for Guangdong Provincial High-level Clinical Key Specialties (No. SZGSP008), Sanming Project of Medicine in Shenzhen (SZSM 202111012, Prof. Guangyan Yu, Oral and Maxillofacial Surgery Team, Peking University Hospital of Stomatology), Research Grants Council (General Research Fund) of Hong Kong, and the Innovation and Technology Commission (ITCPD/17-9) of Hong Kong. Publisher Copyright: © The Author(s) 2023

PY - 2023/2/11

Y1 - 2023/2/11

N2 - In oral squamous cell carcinoma (OSCC), a highly aggressive and frequently lethal malignancy, the role and action mechanism of the microtubule regulatory protein CDK5RAP2 have not been fully understood. Here, we show that CDK5RAP2 is highly expressed in OSCC and its expression correlates with clinical stage and lymph node metastasis of the disease. The expression of CDK5RAP2 is regulated by the Wnt signaling pathway. Depletion of CDK5RAP2 inhibits the tumorigenesis and migration of OSCC cells and alters the OSCC cancer stem (-like) cell (CSC) signature. Notably, suppression of CDK5RAP2 expression disrupts spindle orientation during mitosis. Collectively, these results identify CDK5RAP2 as a potential CSC marker and reveal a mechanism that controls the CSC population in OSCC.

AB - In oral squamous cell carcinoma (OSCC), a highly aggressive and frequently lethal malignancy, the role and action mechanism of the microtubule regulatory protein CDK5RAP2 have not been fully understood. Here, we show that CDK5RAP2 is highly expressed in OSCC and its expression correlates with clinical stage and lymph node metastasis of the disease. The expression of CDK5RAP2 is regulated by the Wnt signaling pathway. Depletion of CDK5RAP2 inhibits the tumorigenesis and migration of OSCC cells and alters the OSCC cancer stem (-like) cell (CSC) signature. Notably, suppression of CDK5RAP2 expression disrupts spindle orientation during mitosis. Collectively, these results identify CDK5RAP2 as a potential CSC marker and reveal a mechanism that controls the CSC population in OSCC.

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DO - 10.1038/s41419-023-05652-z

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VL - 14

JO - Cell Death and Disease

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CDK5RAP2 is a Wnt target gene and promotes stemness and progression of oral squamous cell carcinoma

Abstract

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