Capillary liquid chromatographic-high-resolution mass spectrometric analysis of ribonucleotides

Zongwei CAI*, Fengrui Song, M. S. Yang

*Corresponding author for this work

Research output: Contribution to journalJournal articlepeer-review

27 Citations (Scopus)


A method of capillary HPLC-high-resolution MS was developed for the trace analysis of ATP, GTP, dATP and dGTP. Dimethylhexylamine (DMHA) was used as ion-pairing agent for the HPLC retention and separation of the nucleotides and positive ion electrospray time-of-flight MS was used for the detection. The application of capillary HPLC allowed minimal usage of DMHA while providing excellent peak retention and resolution, which significantly reduced the ion suppression in electrospray ionization-MS analysis and thus increased the sensitivity. Adduct ions of nucleotides and DMHA were used as quantitative ions in order to achieve the best sensitivity. DMHA concentration at 5 mM in the aqueous mobile phase at pH 7 was found to be the optimal conditions for the C18 capillary column. The method was applied to determine ATP level in cultured C6 glioma cells that were treated with toxic concentrations of Zn. The results showed that the cellular ATP level decreased from 2.7 pmol/cell (<10% cell death) in average control cell samples to 0.36 pmol/cell as the concentration of Zn increased to 120 mg/l (>35% cell death) in culture medium.

Original languageEnglish
Pages (from-to)135-143
Number of pages9
JournalJournal of Chromatography A
Issue number1-2
Publication statusPublished - 8 Nov 2002

Scopus Subject Areas

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry

User-Defined Keywords

  • Dimethylhexylamine
  • Ion-pairing reagents
  • Nucleotides
  • Phosphates
  • Ribonucleotides


Dive into the research topics of 'Capillary liquid chromatographic-high-resolution mass spectrometric analysis of ribonucleotides'. Together they form a unique fingerprint.

Cite this