Capillary electrophoresis-ion trap mass spectrometry analysis of Ziagen® and its phosphorylated metabolites

Zongwei CAI*, Eliza N. Fung, Achintya K. Sinhababu

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

17 Citations (Scopus)

Abstract

A capillary electrophoresis-ion trap mass spectrometry method with a time-segment program was developed to simultaneously analyze Ziagen® and its phosphorylated metabolites such as carbovir monophosphate, carbovir diphosphate, and carbovir triphosphate. By using the time-segment program, the positively charged nucleoside analog and negatively charged nucleotides were separated and detected in a single electrophoretic run. The limits of detection were less than 2 μM for all of the analytes. Calibration curves of the compounds showed excellent linearity over the range of 2-100 μM. The capability of the method was demonstrated by analyzing Ziagen® and its phosphorylated metabolites that were spiked in cellular extracts of human peripheral blood mononuclear cells at 20 μM levels. Some endogenous nucleotides such as adenosine 5′-triphosphate, adenosine 5′-diphosphate, and adenosine 5′-monophosphate, were also detected in the cellular extracts.

Original languageEnglish
Pages (from-to)3160-3164
Number of pages5
JournalElectrophoresis
Volume24
Issue number18
DOIs
Publication statusPublished - Sep 2003

Scopus Subject Areas

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry

User-Defined Keywords

  • Capillary electrophoresis-mass spectrometry
  • Endogenous nucleotides
  • Nucleoside analog
  • Phosphorylated metabolites

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