Abstract
The interaction between digestive tract microbiological flora and food
has an important influence on human health. Butyrate is produced during
the fermentation of dietary fibres by intestinal bacteria and plays an
important role in the regulation of mucosal immunity. In this report, we
studied the impact of butyrate on the defence mechanism against the
bacterial membrane component peptidoglycan (PGN). Butyrate was found to
enhance PGN-mediated IL-8 and GRO-α production. The expression of these
chemokines required the activation of NF-κB and was dependent on the
concentrations of butyrate and PGN. Butyrate was found to up-regulate
nucleotide-binding and oligomerisation domain (NOD) 2, but not NOD1 or
TLR2. NOD2 up-regulation was mediated by an increase in histone
acetylation in the Nod2 promoter region, leading to enhanced PGN-induced
IL-8 and GRO-α secretion. Knockdown of NOD2 and TLR2 by siRNA
significantly reduced PGN-mediated chemokine production, suggesting that
both NOD2 and TLR2 are required for maximal response. Our findings
provide a better understanding of the mechanism by which butyrate
regulates mucosal immunity for normal intestinal function. Based on the
results of this study, we infer that dietary fibres can impact
inflammatory bowel diseases.
Original language | English |
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Pages (from-to) | 3529-3537 |
Number of pages | 9 |
Journal | European Journal of Immunology |
Volume | 39 |
Issue number | 12 |
DOIs | |
Publication status | Published - Dec 2009 |
Scopus Subject Areas
- Immunology and Allergy
- Immunology
User-Defined Keywords
- Butyrate
- Intestinal immunity
- Nucleotide-binding and oligomerization domains (NOD)
- Peptidoglycan
- TLR