Bridging the gap between theory and experiment to derive a detailed understanding of hammerhead ribozyme catalysis

Tai Sung Lee, Kin-Yiu WONG, George M. Giambasu, Darrin M. York

Research output: Chapter in book/report/conference proceedingChapterpeer-review

9 Citations (Scopus)

Abstract

Herein we summarize our progress toward the understanding of hammerhead ribozyme (HHR) catalysis through a multiscale simulation strategy. Simulation results collectively paint a picture of HHR catalysis: HHR first folds to form an electronegative active site pocket to recruit a threshold occupation of cationic charges, either a Mg2 + ion or multiple monovalent cations. Catalytically active conformations that have good in-line fitness are supported by specific metal ion coordination patterns that involve either a bridging Mg2 + ion or multiple Na+ ions, one of which is also in a bridging coordination pattern. In the case of a single Mg2 + ion bound in the active site, the Mg2 + ion undergoes a migration that is coupled with deprotonation of the nucleophile (C17:O2'). As the reaction proceeds, the Mg2 + ion stabilizes the accumulating charge of the leaving group and significantly increases the general acid ability of G8:O2'. Further computational mutagenesis simulations suggest that the disruptions due to mutations may severely impact HHR catalysis at different stages of the reaction. Catalytic mechanisms supported by the simulation results are consistent with available structural and biochemical experiments, and together they advance our understanding of HHR catalysis.

Original languageEnglish
Title of host publicationCatalytic RNA
PublisherElsevier B.V.
Pages25-91
Number of pages67
ISBN (Print)9780123812865
DOIs
Publication statusPublished - 2013

Publication series

NameProgress in Molecular Biology and Translational Science
Volume120
ISSN (Print)1877-1173

Scopus Subject Areas

  • Molecular Medicine
  • Molecular Biology

User-Defined Keywords

  • catalysis
  • combined QM/MM
  • enzyme
  • free energy
  • hammerhead ribozyme
  • mechanism
  • RNA
  • simulation

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