TY - JOUR
T1 - Brassinosteroids mediate the effect of soil-drying during meiosis on spikelet degeneration in rice
AU - Zhang, Weiyang
AU - Sheng, Jiayan
AU - Fu, Lidong
AU - Xu, Yunji
AU - Xiong, Fei
AU - Wu, Yunfei
AU - Wang, Weilu
AU - Wang, Zhiqin
AU - ZHANG, Jianhua
AU - Yang, Jianchang
N1 - Funding Information:
We are grateful for grants from National Natural Science Foundation of China ( 31461143015 , 31771710 , 31901445 , 31901444 ), the National Key Research and Development Program of China ( 2016YFD0300206-4 ; 2018YFD0300800 ), the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD) , the Top Talent Supporting Program of Yangzhou University ( 2015-01 ), the Council of Hong Kong Baptist University ( 1806439 ), the Project funded by China Postdoctoral Science Foundation ( 2018M640528 ), and the Open Project from Joint International Research Laboratory of Agriculture and Agri-Product Safety of Yangzhou University ( JRK2018004 ). Appendix A
PY - 2020/1
Y1 - 2020/1
N2 - This study investigated whether and how brassinosteroids (BRs) mediate the effect of soil-drying during meiosis on spikelet degeneration in rice (Oryza sativa L.). A field-grown rice cultivar was exposed to three soil moisture treatments during meiosis: well-watered (WW), moderate soil-drying (MD) and severe soil-drying (SD). Compared with the WW treatment, the MD treatment enhanced BRs biosynthesis, reduced spikelet degeneration, and increased grain yield. The SD treatment had the opposite effects. Changes in the levels of adenosine triphosphate (ATP), energy charge, activities of enzymes involved in energy metabolism, including cytochrome C oxidase (CCO) and succinate dehydrogenase (SDH), and total antioxidant capacity (T-AOC) were consistent with those in BRs levels, whereas the hydrogen peroxide (H2O2) content, vacuolar processing enzyme genes (OsVPE2 and OsVPE3) expression levels and the degree of nuclear DNA fragmentation showed the opposite trend. Knockdown of the BRs synthesis gene OsD11 or application of a BRs biosynthesis inhibitor to young panicles vastly decreased the BRs and ATP contents, energy charge, CCO and SDH activities, and T-AOC in young panicles but increased the H2O2 content, OsVPE2 and OsVPE3 expression, and spikelet degeneration compared to the control, and the opposite effects were observed when exogenous BRs were applied. Therefore, BRs likely mediate the effect of soil-drying on spikelet degeneration, and elevated BRs levels in rice panicles inhibit spikelet degeneration under moderate soil-drying during meiosis by elevating antioxidant capacity and energy status.
AB - This study investigated whether and how brassinosteroids (BRs) mediate the effect of soil-drying during meiosis on spikelet degeneration in rice (Oryza sativa L.). A field-grown rice cultivar was exposed to three soil moisture treatments during meiosis: well-watered (WW), moderate soil-drying (MD) and severe soil-drying (SD). Compared with the WW treatment, the MD treatment enhanced BRs biosynthesis, reduced spikelet degeneration, and increased grain yield. The SD treatment had the opposite effects. Changes in the levels of adenosine triphosphate (ATP), energy charge, activities of enzymes involved in energy metabolism, including cytochrome C oxidase (CCO) and succinate dehydrogenase (SDH), and total antioxidant capacity (T-AOC) were consistent with those in BRs levels, whereas the hydrogen peroxide (H2O2) content, vacuolar processing enzyme genes (OsVPE2 and OsVPE3) expression levels and the degree of nuclear DNA fragmentation showed the opposite trend. Knockdown of the BRs synthesis gene OsD11 or application of a BRs biosynthesis inhibitor to young panicles vastly decreased the BRs and ATP contents, energy charge, CCO and SDH activities, and T-AOC in young panicles but increased the H2O2 content, OsVPE2 and OsVPE3 expression, and spikelet degeneration compared to the control, and the opposite effects were observed when exogenous BRs were applied. Therefore, BRs likely mediate the effect of soil-drying on spikelet degeneration, and elevated BRs levels in rice panicles inhibit spikelet degeneration under moderate soil-drying during meiosis by elevating antioxidant capacity and energy status.
KW - Antioxidant capacity
KW - Brassinosteroids (BRs)
KW - Energy status
KW - Rice (Oryza sativaL.)
KW - Soil-drying
KW - Spikelet degeneration
UR - http://www.scopus.com/inward/record.url?scp=85074338811&partnerID=8YFLogxK
U2 - 10.1016/j.envexpbot.2019.103887
DO - 10.1016/j.envexpbot.2019.103887
M3 - Journal article
AN - SCOPUS:85074338811
SN - 0098-8472
VL - 169
JO - Environmental and Experimental Botany
JF - Environmental and Experimental Botany
M1 - 103887
ER -