TY - JOUR
T1 - Brassinosteroids mediate the effect of high temperature during anthesis on the pistil activity of photo-thermosensitive genetic male-sterile rice lines
AU - Chen, Jing
AU - Fei, Keqi
AU - Zhang, Weiyang
AU - Wang, Zhiqin
AU - Zhang, Jianhua
AU - Yang, Jianchang
N1 - Funding Information:
This work was supported by the National Natural Science Foundation of China ( 31771710 , 31901445 ), the National Key Research and Development Program of China ( 2016YFD0300206-4 , 2018YFD0300800 ), the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD), and the Top Talent Supporting Program of Yangzhou University ( 2015-01 ).
Publisher Copyright:
© 2020
PY - 2021/2
Y1 - 2021/2
N2 - Brassinosteroids (BRs) play critical roles in a wide range of plant developmental processes. However, it is unknown whether and how BRs mediate the effect of high temperature (HT) stress during anthesis on the pistil activity of photo-thermosensitive genetic male-sterile (PTSGMS) rice (Oryza sativa L.) lines. This study investigated the question. Three pot-grown PTSGMS rice lines were subjected to HT stress during anthesis. The contents of 24-epibrassinolide (24-EBL) and 28-homobrassinolide (28-HBL), the major forms of BR in rice plants, and levels of reactive oxygen species (ROS) or antioxidants (AOS), hydrogen peroxide (H2O2), 1-aminocylopropane-1-carboxylic acid (ACC), ascorbic acid (AsA), and catalase activity in pistils, were determined. HT stress significantly reduced the contents of both 24-EBL and 28-EBL relative to those under normal temperatures, but the reduction varied by PTSGMS line. A line with higher BR contents under HT stress showed lower contents of ACC and H2O2, higher catalase activity and AsA content in pistils, and higher fertilization rate, seed-setting rate, and seed yield when the line was crossed with a restorer line, indicating that higher levels of BRs increase HT stress resistance. Applying 24-EBL, 28-HBL or an inhibitor of BR biosynthesis confirmed the roles of BRs in response to HT stress. The results suggest that BRs mediate the effect of HT stress on pistil activity during anthesis and alleviate the harm of HT stress by increasing AOS and suppressing ROS generation.
AB - Brassinosteroids (BRs) play critical roles in a wide range of plant developmental processes. However, it is unknown whether and how BRs mediate the effect of high temperature (HT) stress during anthesis on the pistil activity of photo-thermosensitive genetic male-sterile (PTSGMS) rice (Oryza sativa L.) lines. This study investigated the question. Three pot-grown PTSGMS rice lines were subjected to HT stress during anthesis. The contents of 24-epibrassinolide (24-EBL) and 28-homobrassinolide (28-HBL), the major forms of BR in rice plants, and levels of reactive oxygen species (ROS) or antioxidants (AOS), hydrogen peroxide (H2O2), 1-aminocylopropane-1-carboxylic acid (ACC), ascorbic acid (AsA), and catalase activity in pistils, were determined. HT stress significantly reduced the contents of both 24-EBL and 28-EBL relative to those under normal temperatures, but the reduction varied by PTSGMS line. A line with higher BR contents under HT stress showed lower contents of ACC and H2O2, higher catalase activity and AsA content in pistils, and higher fertilization rate, seed-setting rate, and seed yield when the line was crossed with a restorer line, indicating that higher levels of BRs increase HT stress resistance. Applying 24-EBL, 28-HBL or an inhibitor of BR biosynthesis confirmed the roles of BRs in response to HT stress. The results suggest that BRs mediate the effect of HT stress on pistil activity during anthesis and alleviate the harm of HT stress by increasing AOS and suppressing ROS generation.
KW - Brassinosteroids
KW - High temperature
KW - Photo-thermosensitive genetic male-sterile (PTSGMS)
KW - Pistil activity
KW - Rice
UR - http://www.scopus.com/inward/record.url?scp=85090989211&partnerID=8YFLogxK
U2 - 10.1016/j.cj.2020.07.001
DO - 10.1016/j.cj.2020.07.001
M3 - Journal article
AN - SCOPUS:85090989211
SN - 2095-5421
VL - 9
SP - 109
EP - 119
JO - Crop Journal
JF - Crop Journal
IS - 1
ER -