Bovine serum albumin-confined silver nanoclusters as fluorometric probe for detection of biothiols

Zhen Chen, Dongtao Lu, Zongwei CAI, Chuan Dong, Shaomin Shuang*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

53 Citations (Scopus)

Abstract

Fluorescent bovine serum albumin-confined silver nanoclusters (BSA-AgNCs) were demonstrated to be a novel and environmentally friendly probe for the rapid detection of biothiols such as cysteine (Cys), homocysteine (Hcy) and glutathione (GSH). The sensing was ascribed to the strong affinity between the mercapto group of the biothiols and the silver nanoclusters. The fluorescence intensity of BSA-AgNCs was quenched efficiently on increasing the concentration of biothiol, corresponding with a red-shift in emission wavelength. However, the fluorescence of the silver nanoclusters was almost unchanged in the presence of other α-amino acids at 10-fold higher concentrations. By virtue of this specific response, a new, simple and rapid fluorescent method for detecting biothiols has been developed. The linear ranges for Cys, Hcy and GSH were 2.0 × 10-6to 9.0 × 10-5M (R2= 0.994), 2.0 × 10-6to 1.2 × 10-4M (R2= 0.996) and 1.0 × 10-5to 8.0 × 10-5M (R2= 0.980), respectively. The detection limits were 8.1 × 10-7M for Cys, 1.0 × 10-6M for Hcy and 1.1 × 10-6M for GSH. Our proposed method was successfully applied to the determination of thiols in human plasma and the recovery was 94.83-105.24%. It is potentially applicable to protein-stabilized silver nanoclusters in a chemical or biochemical sensing system.

Original languageEnglish
Pages (from-to)722-727
Number of pages6
JournalLuminescence
Volume29
Issue number7
DOIs
Publication statusPublished - 1 Nov 2014

Scopus Subject Areas

  • Biophysics
  • Chemistry (miscellaneous)

User-Defined Keywords

  • Biothiols
  • Bovine serum albumin
  • Fluorescence
  • Silver nanoclusters

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