Bone marrow cells from A/J mice do not proliferate in interleukin‐3 but express normal numbers of interleukin‐3 receptors

Andrew J. Hapel*, Ming‐Chiu ‐C Fung, Nai Ki Mak, Carolyn Morris, Donald Metcalf, Nicos Nicola

*Corresponding author for this work

Research output: Contribution to journalJournal articlepeer-review

3 Citations (Scopus)

Abstract

Summary. Haemopoietic cells from A/J mice do not form colonies (proliferate) in response to interleukin‐3 (multi‐CSF. IL‐3). We have examined different populations of cells from A/J mice and shown that, despite their failure to proliferate in response to IL‐3, cells from bone marrow, spleen and the peritoneum all bound 125I‐labelled IL‐3. A wide variety of cell types bound IL‐3 as determined by autoradiography, including promyelocytes, myelocytes, metamyelocytes, polymorphs, promonocytes, monocytes, eosinophils and lymphocytes, but not nucleated erythroid cells, and the proportion of each cell type binding label was similar when cells from A/J mice were compared with those of C57B1/6 and Balb/c mice. Bone marrow cells from A/J mice internalized interleukin‐3 with normal kinetics and mRNA extracted from these cells contains the same species of IL‐3 receptor and IL‐3 receptor‐like mRNAs as are found in the other strains. Collectively the data suggest that the failure of haemopoietic cells from A/J mice to proliferate in response to IL‐3 is related to a selective defect in signalling to proliferation specific genes. This defect is apparently not related to internalization or processing of the IL‐3/IL‐3‐receptor complex, but may be due to failure to activate appropriate accessory molecules in the cell.

Original languageEnglish
Pages (from-to)488-493
Number of pages6
JournalBritish Journal of Haematology
Volume82
Issue number3
DOIs
Publication statusPublished - Nov 1992

Scopus Subject Areas

  • Hematology

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