TY - JOUR
T1 - Bone marrow cells from A/J mice do not proliferate in interleukin‐3 but express normal numbers of interleukin‐3 receptors
AU - Hapel, Andrew J.
AU - Fung, Ming‐Chiu ‐C
AU - Mak, Nai Ki
AU - Morris, Carolyn
AU - Metcalf, Donald
AU - Nicola, Nicos
N1 - Copyright:
Copyright 2016 Elsevier B.V., All rights reserved.
PY - 1992/11
Y1 - 1992/11
N2 - Summary. Haemopoietic cells from A/J mice do not form colonies (proliferate) in response to interleukin‐3 (multi‐CSF. IL‐3). We have examined different populations of cells from A/J mice and shown that, despite their failure to proliferate in response to IL‐3, cells from bone marrow, spleen and the peritoneum all bound 125I‐labelled IL‐3. A wide variety of cell types bound IL‐3 as determined by autoradiography, including promyelocytes, myelocytes, metamyelocytes, polymorphs, promonocytes, monocytes, eosinophils and lymphocytes, but not nucleated erythroid cells, and the proportion of each cell type binding label was similar when cells from A/J mice were compared with those of C57B1/6 and Balb/c mice. Bone marrow cells from A/J mice internalized interleukin‐3 with normal kinetics and mRNA extracted from these cells contains the same species of IL‐3 receptor and IL‐3 receptor‐like mRNAs as are found in the other strains. Collectively the data suggest that the failure of haemopoietic cells from A/J mice to proliferate in response to IL‐3 is related to a selective defect in signalling to proliferation specific genes. This defect is apparently not related to internalization or processing of the IL‐3/IL‐3‐receptor complex, but may be due to failure to activate appropriate accessory molecules in the cell.
AB - Summary. Haemopoietic cells from A/J mice do not form colonies (proliferate) in response to interleukin‐3 (multi‐CSF. IL‐3). We have examined different populations of cells from A/J mice and shown that, despite their failure to proliferate in response to IL‐3, cells from bone marrow, spleen and the peritoneum all bound 125I‐labelled IL‐3. A wide variety of cell types bound IL‐3 as determined by autoradiography, including promyelocytes, myelocytes, metamyelocytes, polymorphs, promonocytes, monocytes, eosinophils and lymphocytes, but not nucleated erythroid cells, and the proportion of each cell type binding label was similar when cells from A/J mice were compared with those of C57B1/6 and Balb/c mice. Bone marrow cells from A/J mice internalized interleukin‐3 with normal kinetics and mRNA extracted from these cells contains the same species of IL‐3 receptor and IL‐3 receptor‐like mRNAs as are found in the other strains. Collectively the data suggest that the failure of haemopoietic cells from A/J mice to proliferate in response to IL‐3 is related to a selective defect in signalling to proliferation specific genes. This defect is apparently not related to internalization or processing of the IL‐3/IL‐3‐receptor complex, but may be due to failure to activate appropriate accessory molecules in the cell.
UR - http://www.scopus.com/inward/record.url?scp=0026486908&partnerID=8YFLogxK
U2 - 10.1111/j.1365-2141.1992.tb06457.x
DO - 10.1111/j.1365-2141.1992.tb06457.x
M3 - Journal article
C2 - 1486029
AN - SCOPUS:0026486908
SN - 0007-1048
VL - 82
SP - 488
EP - 493
JO - British Journal of Haematology
JF - British Journal of Haematology
IS - 3
ER -