TY - JOUR
T1 - Bisphenol S induced epigenetic and transcriptional changes in human breast cancer cell line MCF-7
AU - Huang, Wei
AU - Zhao, Chao
AU - Zhong, Huan
AU - ZHANG, Shoudong
AU - Xia, Yiji
AU - Cai, Zongwei
N1 - Funding Information:
This work was supported by National Natural Science Foundation of China ( 21507106 , 21806135 and 91543202 ), Hong Kong Research Grants Council-General Research Fund ( 1230195 ), Hong Kong Baptist University Strategic Development Fund ( 15-1012-P04 ) and State-Key Laboratory Research Grant ( SKLP-1617-P02 ), Hong Kong Scholars Program Fund ( XJ2016010 ).
PY - 2019/3
Y1 - 2019/3
N2 - In recent years, concerns about using Bisphenol A (BPA) in daily consume products and its effects in many chronic human diseases have prompted the removal of BPA. However, the widely used BPA alternatives, including Bisphenol S (BPS), have a high structural similarity with BPA, suggesting that they may have similar biological effects towards human beings. Indeed, BPS was also found to have endocrine-disrupting effects. Epigenetic mechanism was reported to be involved in BPA-induced biological effects in both in vitro and in vivo models. However, there is no assessment on whether BPS could cause epigenetic changes. In this work, we investigated the possible epigenetic effects of BPS that might induce in human breast cancer cell line MCF-7. We found that BPS could change DNA methylation level of transposons. Besides, methylation status in promoter of breast cancer related genes CDH1, SFN, TNFRSF10C were also changed, which implied that BPS might play a role in the development of breast cancer. Gene expression profiling showed that some genes related to breast cancer progression were upregulated, including THBS4, PPARGC1A, CREB5, COL5A3. Gene ontology (GO) analysis of the differentially expressed genes revealed the significantly changes in PI3K-Akt signaling pathway and extracellular matrix, which were related to the proliferation, migration and invasion of breast cancer cells. These results illustrated that BPS exposure might play roles in the progression of breast cancer.
AB - In recent years, concerns about using Bisphenol A (BPA) in daily consume products and its effects in many chronic human diseases have prompted the removal of BPA. However, the widely used BPA alternatives, including Bisphenol S (BPS), have a high structural similarity with BPA, suggesting that they may have similar biological effects towards human beings. Indeed, BPS was also found to have endocrine-disrupting effects. Epigenetic mechanism was reported to be involved in BPA-induced biological effects in both in vitro and in vivo models. However, there is no assessment on whether BPS could cause epigenetic changes. In this work, we investigated the possible epigenetic effects of BPS that might induce in human breast cancer cell line MCF-7. We found that BPS could change DNA methylation level of transposons. Besides, methylation status in promoter of breast cancer related genes CDH1, SFN, TNFRSF10C were also changed, which implied that BPS might play a role in the development of breast cancer. Gene expression profiling showed that some genes related to breast cancer progression were upregulated, including THBS4, PPARGC1A, CREB5, COL5A3. Gene ontology (GO) analysis of the differentially expressed genes revealed the significantly changes in PI3K-Akt signaling pathway and extracellular matrix, which were related to the proliferation, migration and invasion of breast cancer cells. These results illustrated that BPS exposure might play roles in the progression of breast cancer.
KW - Bisphenol S
KW - Breast cancer cell
KW - DNA methylation
KW - Gene expression profiling
UR - http://www.scopus.com/inward/record.url?scp=85060043627&partnerID=8YFLogxK
U2 - 10.1016/j.envpol.2018.12.084
DO - 10.1016/j.envpol.2018.12.084
M3 - Journal article
C2 - 30616060
AN - SCOPUS:85060043627
SN - 0269-7491
VL - 246
SP - 697
EP - 703
JO - Environmental Pollution
JF - Environmental Pollution
ER -
