Biotransformation of Tetrabromobisphenol A and Its Analogs by Selected Gut Bacteria Strains: Implications for Human Health

Meilin Lv, Yanna Liu*, Minghao Wang, Yi Wang, Tongtong Xiang, Yunhe Guo, Xue Chao Song, Yuhao Yan, Jie Gao, Chunzhen Shi, Wenxiao Pan, Aifeng Liu, Linlin Yao, Xiliang Yan, Liqun Chen, Runzeng Liu, Jianbo Shi, Bing Yan, Zongwei Cai, Guangbo Qu*Guibin Jiang

*Corresponding author for this work

Research output: Contribution to journalJournal articlepeer-review

Abstract

Knowledge of the biotransformation of tetrabromobisphenol A (TBBPA) and its related contaminants by human gut microbiota (GM) remains unexplored. Here, TBBPA and its four analogs were incubated with mixed GM strains, and nine rhamnosylated or debrominated transformation products (TPs) were discovered. Remarkably, rhamnosylation was identified as a common and unique microbial transformation pathway for these contaminants, and six of the seven rhamnosylated TPs were reported for the first time. Additionally, a kinetic transformation study also showed a rapid and strong bioaccumulation of TBBPA and TPs by Clostridium manihotivorum. Genomic analysis and phylogenetic studies identified C1.1_02053 as the gene encoding the C. manihotivorum working rhamnosyltransferase (CmRT), showing elevated gene expression with higher TBBPA exposure. Molecular docking identified five critical amino acid residues in CmRT that catalyze TBBPA rhamnosylation, and molecular dynamics simulations further confirmed the stability of the CmRT-TBBPA complex. Dynamic metabolomics analysis showed microbial growth-dependent disturbing effects in C. manihotivorum upon TBBPA exposure, and key metabolic pathways related to rhamnosyltransferase showed changes closely related to the transformation process. These findings provide insights into the unique transformation of environmental contaminants by the GM and highlight the disturbing effects of exogenous chemicals on the GM, as well as the potential impacts on overall human health.

Original languageEnglish
Pages (from-to)20894-20905
Number of pages12
JournalEnvironmental Science and Technology
Volume58
Issue number47
DOIs
Publication statusPublished - 26 Nov 2024

Scopus Subject Areas

  • General Chemistry
  • Environmental Chemistry

User-Defined Keywords

  • biotransformation
  • genomics
  • gut microbiota
  • metabolomics
  • rhamnosylation
  • TBBPA

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