TY - JOUR
T1 - Biotechnological Exploration of Transformed Root Culture for Value-Added Products
AU - Shi, Min
AU - Liao, Pan
AU - Nile, Shivraj Hariram
AU - Georgiev, Milen I.
AU - Kai, Guoyin
N1 - Funding Information:
This work was funded by National Natural Science Fund of China (81522049, 1571735, 31270007), Zhejiang Provincial Ten Thousands Program for Leading Talents of Science and Technology Innovation (2018R52050), Zhejiang Provincial Program for the Cultivation of High-level Innovative Health talents, Shanghai Science and Technology Committee Project (17JC1404300, 15430502700) and the ‘Dawn’ Program of Shanghai Education Commission (16SG38). M.I.G. acknowledges the financial support of the European’s Union Horizon 2020 Research and Innovation Programme, project PlantaSYST (SGA-CSA No. 739582 under FPA No. 664620).
Publisher Copyright:
2020 The Author(s). Published by Elsevier Ltd.
PY - 2021/2
Y1 - 2021/2
N2 - Medicinal plants produce valuable secondary metabolites with anticancer,
analgesic, anticholinergic or other activities, but low metabolite
levels and limited available tissue restrict metabolite yields.
Transformed root cultures, also called hairy roots, provide a feasible
approach for producing valuable secondary metabolites. Various
strategies have been used to enhance secondary metabolite production in
hairy roots, including increasing substrate availability, regulating key
biosynthetic genes, multigene engineering, combining genetic
engineering and elicitation, using transcription factors (TFs), and
introducing new genes. In this review, we focus on recent developments
in hairy roots from medicinal plants, techniques to boost production of
desired secondary metabolites, and the development of new technologies
to study these metabolites. We also discuss recent trends, emerging
applications, and future perspectives.
AB - Medicinal plants produce valuable secondary metabolites with anticancer,
analgesic, anticholinergic or other activities, but low metabolite
levels and limited available tissue restrict metabolite yields.
Transformed root cultures, also called hairy roots, provide a feasible
approach for producing valuable secondary metabolites. Various
strategies have been used to enhance secondary metabolite production in
hairy roots, including increasing substrate availability, regulating key
biosynthetic genes, multigene engineering, combining genetic
engineering and elicitation, using transcription factors (TFs), and
introducing new genes. In this review, we focus on recent developments
in hairy roots from medicinal plants, techniques to boost production of
desired secondary metabolites, and the development of new technologies
to study these metabolites. We also discuss recent trends, emerging
applications, and future perspectives.
KW - biosynthesis
KW - biotechnology
KW - genome editing
KW - hairy roots
KW - secondary metabolites
UR - http://www.scopus.com/inward/record.url?scp=85088096332&partnerID=8YFLogxK
U2 - 10.1016/j.tibtech.2020.06.012
DO - 10.1016/j.tibtech.2020.06.012
M3 - Review article
C2 - 32690221
AN - SCOPUS:85088096332
SN - 0167-7799
VL - 39
SP - 137
EP - 149
JO - Trends in Biotechnology
JF - Trends in Biotechnology
IS - 2
ER -
