TY - JOUR
T1 - Biosensors for determination of glucose with glucose oxidase immobilized on an eggshell membrane
AU - Wu, Baoli
AU - Zhang, Guomei
AU - Shuang, Shaomin
AU - Choi, Martin M.F.
N1 - Funding Information:
This work is supported by the National Natural Science Foundation of China (grant no. 20172035). The National Education Committee Foundation for Outstanding Young teachers and the Natural Science Foundation of Shanxi Province are also gratefully acknowledged.
PY - 2004/10/8
Y1 - 2004/10/8
N2 - A glucose biosensor using an enzyme-immobilized eggshell membrane and oxygen electrode for glucose determination has been fabricated. Glucose oxidase was covalently immobilized on an eggshell membrane with glutaraldehyde as a cross-linking agent. The glucose biosensor was fabricated by positioning the enzyme-immobilized eggshell membrane on the surface of a dissolved oxygen sensor. The detection scheme was based on the depletion of dissolved oxygen content upon exposure to glucose solution and the decrease in the oxygen level was monitored and related to the glucose concentration. The effect of glutaraldehyde concentration, pH, phosphate buffer concentration and temperature on the response of the glucose biosensor has been studied in detail. Common matrix interferents such as ethanol, D-fructose, citric acid, sodium benzoate, sucrose and L-ascorbic acid did not give significant interference. The resulting sensor exhibited a fast response (100 s), high sensitivity (8.3409 mg L -1 oxygen depletion/mmol L-1 glucose) and good storage stability (85.2% of its initial sensitivity after 4 months). The linear response is 1.0×10-5 to 1.3×10-3 mol L-1 glucose. The glucose content in real samples such as commercial glucose injection preparations and wines was determined, and the results were comparable to the values obtained from a commercial glucose assay kit based on a spectrophotometric method.
AB - A glucose biosensor using an enzyme-immobilized eggshell membrane and oxygen electrode for glucose determination has been fabricated. Glucose oxidase was covalently immobilized on an eggshell membrane with glutaraldehyde as a cross-linking agent. The glucose biosensor was fabricated by positioning the enzyme-immobilized eggshell membrane on the surface of a dissolved oxygen sensor. The detection scheme was based on the depletion of dissolved oxygen content upon exposure to glucose solution and the decrease in the oxygen level was monitored and related to the glucose concentration. The effect of glutaraldehyde concentration, pH, phosphate buffer concentration and temperature on the response of the glucose biosensor has been studied in detail. Common matrix interferents such as ethanol, D-fructose, citric acid, sodium benzoate, sucrose and L-ascorbic acid did not give significant interference. The resulting sensor exhibited a fast response (100 s), high sensitivity (8.3409 mg L -1 oxygen depletion/mmol L-1 glucose) and good storage stability (85.2% of its initial sensitivity after 4 months). The linear response is 1.0×10-5 to 1.3×10-3 mol L-1 glucose. The glucose content in real samples such as commercial glucose injection preparations and wines was determined, and the results were comparable to the values obtained from a commercial glucose assay kit based on a spectrophotometric method.
KW - Eggshell membrane
KW - Glucose biosensor
KW - Glucose oxidase
UR - http://www.scopus.com/inward/record.url?scp=4143141128&partnerID=8YFLogxK
U2 - 10.1016/j.talanta.2004.03.050
DO - 10.1016/j.talanta.2004.03.050
M3 - Journal article
AN - SCOPUS:4143141128
SN - 0039-9140
VL - 64
SP - 546
EP - 553
JO - Talanta
JF - Talanta
IS - 2
ER -