Biosensors for determination of galactose with galactose oxidase immobilized on eggshell membrane

Guangming Wen, Yan Zhang, Yehong Zhou, Shaomin Shuang*, Chuan Dong, Martin M F CHOI

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

12 Citations (Scopus)

Abstract

A galactose biosensor was first fabricated using a galactose oxidase-immobilized eggshell membrane and an oxygen electrode. The detection scheme was based on the depletion of dissolved oxygen content upon exposure to galactose solution, and the decrease in oxygen level was monitored and related to the galactose concentration. The effects of pH, buffer compositions, phosphate buffer concentration, and temperature on the biosensor were investigated. The linear range is 0.1 to 8.5 mM with relative standard deviation (RSD) is 3.74% and the response time is 100 s. Some possible interferents such as L-ascorbic acid, ethanol, sucrose, glucose, maltose, and fructose did not give significant interference and retained 83.6% of initial enzyme activity after 3 months when stored in a pH 7.0 phosphate buffer solution at 4°C. The proposed biosensor was applied to the determination of galactose added to plasma samples with the recoveries of galactose is 94%-110%.

Original languageEnglish
Pages (from-to)1519-1529
Number of pages11
JournalAnalytical Letters
Volume38
Issue number10
DOIs
Publication statusPublished - 2005

Scopus Subject Areas

  • Analytical Chemistry
  • Biochemistry
  • Spectroscopy
  • Clinical Biochemistry
  • Biochemistry, medical
  • Electrochemistry

User-Defined Keywords

  • Eggshell membrane
  • Galactose biosensor
  • Galactose oxidase

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