Biosensor for determination of hydrogen peroxide based on Yucca filamentosa membrane

Huan Yue; Jie He; Dan Xiao; Martin M F CHOI

doi:10.1039/c3ay40678d

Biosensor for determination of hydrogen peroxide based on Yucca filamentosa membrane

Huan Yue, Jie He, Dan Xiao^*, Martin M F CHOI

^*Corresponding author for this work

Department of Chemistry

Research output: Contribution to journal › Journal article › peer-review

3 Citations (Scopus)

Abstract

A simple and low-cost hydrogen peroxide (H₂O₂) biosensor has been developed using a Yucca filamentosa plant leaf membrane in conjunction with an oxygen (O₂) sensor. The leaf membrane contains hydrogen peroxidase which can catalytically decompose H₂O₂ to produce O₂. The response rate was faster for a Yucca membrane (t₉₀ response time is ≈14 s) than a Yucca membrane with the O ₂-permeable membrane (t₉₀ response time is ≈200 s) and the sensitivity was much improved. The biosensor exhibited an excellent linear calibration range from 85 μM to 3.75 mM H₂O₂ (correlation coefficient r = 0.9999) with a detection limit of 15.1 μM; repeatability (1.024%, n = 10). The effects of pH and temperature on the response of the H₂O₂ biosensor have been studied in detail. The working life-time of the biosensor was good as it retains 86.7% of its initial sensitivity at 25 °C even after 2350 determinations of H ₂O₂ sample solutions. It has been successfully applied to the determination of H₂O₂ concentration in some commercial samples.

Original language	English
Pages (from-to)	5437-5443
Number of pages	7
Journal	Analytical Methods
Volume	5
Issue number	20
DOIs	https://doi.org/10.1039/c3ay40678d
Publication status	Published - 21 Oct 2013

Scopus Subject Areas

Analytical Chemistry
Chemical Engineering(all)
Engineering(all)

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title = "Biosensor for determination of hydrogen peroxide based on Yucca filamentosa membrane",

abstract = "A simple and low-cost hydrogen peroxide (H2O2) biosensor has been developed using a Yucca filamentosa plant leaf membrane in conjunction with an oxygen (O2) sensor. The leaf membrane contains hydrogen peroxidase which can catalytically decompose H2O2 to produce O2. The response rate was faster for a Yucca membrane (t90 response time is ≈14 s) than a Yucca membrane with the O 2-permeable membrane (t90 response time is ≈200 s) and the sensitivity was much improved. The biosensor exhibited an excellent linear calibration range from 85 μM to 3.75 mM H2O2 (correlation coefficient r = 0.9999) with a detection limit of 15.1 μM; repeatability (1.024%, n = 10). The effects of pH and temperature on the response of the H2O2 biosensor have been studied in detail. The working life-time of the biosensor was good as it retains 86.7% of its initial sensitivity at 25 °C even after 2350 determinations of H 2O2 sample solutions. It has been successfully applied to the determination of H2O2 concentration in some commercial samples.",

author = "Huan Yue and Jie He and Dan Xiao and CHOI, {Martin M F}",

year = "2013",

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TY - JOUR

T1 - Biosensor for determination of hydrogen peroxide based on Yucca filamentosa membrane

AU - Yue, Huan

AU - He, Jie

AU - Xiao, Dan

AU - CHOI, Martin M F

PY - 2013/10/21

Y1 - 2013/10/21

N2 - A simple and low-cost hydrogen peroxide (H2O2) biosensor has been developed using a Yucca filamentosa plant leaf membrane in conjunction with an oxygen (O2) sensor. The leaf membrane contains hydrogen peroxidase which can catalytically decompose H2O2 to produce O2. The response rate was faster for a Yucca membrane (t90 response time is ≈14 s) than a Yucca membrane with the O 2-permeable membrane (t90 response time is ≈200 s) and the sensitivity was much improved. The biosensor exhibited an excellent linear calibration range from 85 μM to 3.75 mM H2O2 (correlation coefficient r = 0.9999) with a detection limit of 15.1 μM; repeatability (1.024%, n = 10). The effects of pH and temperature on the response of the H2O2 biosensor have been studied in detail. The working life-time of the biosensor was good as it retains 86.7% of its initial sensitivity at 25 °C even after 2350 determinations of H 2O2 sample solutions. It has been successfully applied to the determination of H2O2 concentration in some commercial samples.

AB - A simple and low-cost hydrogen peroxide (H2O2) biosensor has been developed using a Yucca filamentosa plant leaf membrane in conjunction with an oxygen (O2) sensor. The leaf membrane contains hydrogen peroxidase which can catalytically decompose H2O2 to produce O2. The response rate was faster for a Yucca membrane (t90 response time is ≈14 s) than a Yucca membrane with the O 2-permeable membrane (t90 response time is ≈200 s) and the sensitivity was much improved. The biosensor exhibited an excellent linear calibration range from 85 μM to 3.75 mM H2O2 (correlation coefficient r = 0.9999) with a detection limit of 15.1 μM; repeatability (1.024%, n = 10). The effects of pH and temperature on the response of the H2O2 biosensor have been studied in detail. The working life-time of the biosensor was good as it retains 86.7% of its initial sensitivity at 25 °C even after 2350 determinations of H 2O2 sample solutions. It has been successfully applied to the determination of H2O2 concentration in some commercial samples.

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U2 - 10.1039/c3ay40678d

DO - 10.1039/c3ay40678d

M3 - Journal article

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SN - 1759-9660

VL - 5

SP - 5437

EP - 5443

JO - Analytical Methods

JF - Analytical Methods

IS - 20

ER -

Biosensor for determination of hydrogen peroxide based on Yucca filamentosa membrane

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