TY - JOUR
T1 - Biological and microcalorimetric studies of the toxic effect of organoarsenic(V) compounds to wild strain of Bacillus thuringiensis
AU - Russel, Mohammad
AU - Yao, Jun
AU - Chen, Huilun
AU - Wang, Fei
AU - Zhou, Yong
AU - Zhuang, Rensheng
AU - Choi, Martin M.F.
AU - Ceccanti, Brunello
AU - Trebse, Polonca
AU - Zaray, Gyula
N1 - Funding Information:
Acknowledgments This work was supported in part by grants from the Sino-Italian, Sino-German PPP, Sino-Hungarian, Sino-Slovenian Governmental International Scientific and Technological Cooperation Project (200625, 20063139, 2006294, and 2007203), National Natural Science Foundation of China (40425001 and 40673065), the Specialized Research Fund for the Doctoral Program of Higher Education (20060491508), the Key Project of Chinese Ministry of Education (107077), the Hubei Key International Cooperation Project (2006CA007), and 111 project (B08030).
PY - 2009/11
Y1 - 2009/11
N2 - Microcalorimetric and biological methods were carried out to determine the toxicity of dimethylarsinic acid (DMA) and monomethylarsonic acid (MMA) to wild strain of Bacillus thuringiensis. Thermokinetic parameters were obtained from the power-time curves, showing that the peak-heat output power, total heat output, and number of colonies decreased with the increases in concentration of DMA and MMA. In addition, the generation time and peak maximal time increased with the increases in the dosage of DMA and MMA. The half inhibitory concentrations of DMA and MMA were 99.02 and 142.02 λ.gmL-1, respectively for the wild strain of B. thuringiensis. DMA shows higher toxicity to bacteria than MMA. The toxicity resistance of B. thuringiensis against organoarsenic(V) is quite high for the wild strain. Our work demonstrates that micro-calorimetry is a very sensitive, simple, and useful technique for in vitro investigation of the toxic effect of organoarsenic(V) on microbial activity.
AB - Microcalorimetric and biological methods were carried out to determine the toxicity of dimethylarsinic acid (DMA) and monomethylarsonic acid (MMA) to wild strain of Bacillus thuringiensis. Thermokinetic parameters were obtained from the power-time curves, showing that the peak-heat output power, total heat output, and number of colonies decreased with the increases in concentration of DMA and MMA. In addition, the generation time and peak maximal time increased with the increases in the dosage of DMA and MMA. The half inhibitory concentrations of DMA and MMA were 99.02 and 142.02 λ.gmL-1, respectively for the wild strain of B. thuringiensis. DMA shows higher toxicity to bacteria than MMA. The toxicity resistance of B. thuringiensis against organoarsenic(V) is quite high for the wild strain. Our work demonstrates that micro-calorimetry is a very sensitive, simple, and useful technique for in vitro investigation of the toxic effect of organoarsenic(V) on microbial activity.
KW - Bacillus thuringiensis
KW - Half inhibitory doses
KW - Microcalorimetry
KW - Optical density
KW - Toxicity
UR - http://www.scopus.com/inward/record.url?scp=73949134061&partnerID=8YFLogxK
U2 - 10.1007/s12011-009-8358-1
DO - 10.1007/s12011-009-8358-1
M3 - Journal article
C2 - 19294342
AN - SCOPUS:73949134061
SN - 0163-4984
VL - 131
SP - 192
EP - 203
JO - Biological Trace Element Research
JF - Biological Trace Element Research
IS - 2
ER -
