Bioinspired construction of histidine-doped porphyrin covalent organic framework nanozyme with enhanced peroxidase-like activity for sensitive uric acid detection

Chao Zhong; Cong Hu; Dan Ouyang; Akang Dan; Yanhui Zhong; Zongwei Cai; Zian Lin

doi:10.1016/j.cej.2023.146979

Bioinspired construction of histidine-doped porphyrin covalent organic framework nanozyme with enhanced peroxidase-like activity for sensitive uric acid detection

Chao Zhong, Cong Hu, Dan Ouyang, Akang Dan, Yanhui Zhong^*, Zongwei Cai, Zian Lin^*

^*Corresponding author for this work

Research output: Contribution to journal › Journal article › peer-review

44 Citations (Scopus)

Abstract

Imitating catalytic sites of natural enzymes is a promising approach to accurately improve the catalytic performances of nanozymes. Herein, inspired by the structure of natural horseradish peroxidase (HRP), three-component condensation strategy is unitized to design histidine (His) functionalized iron porphyrin covalent organic frameworks (Fe-COF-H3) nanozyme with peroxidase-like (POD-like) activity. The introduced His could form favorable microenvironment around the iron porphyrin structure, enhancing the catalytic capacity of COF nanozyme. Taking advantage of its excellent POD-like activity, Fe-COF-H3 was utilized as a carrier to immobilize urate oxidase (UOx) for the construction of enzyme-nanozyme cascade system (UOx@Fe-COF-H3). The UOx@Fe-COF-H3 was successfully applied for the colorimetric detection of uric acid (UA) in biological samples. This work not only offers a new insight to develop high-efficiency COF nanozymes, but also expands the application of COF nanozyme for the construction of enzyme-nanozyme cascade system.

Original language	English
Article number	146979
Journal	Chemical Engineering Journal
Volume	477
Early online date	28 Oct 2023
DOIs	https://doi.org/10.1016/j.cej.2023.146979
Publication status	Published - 1 Dec 2023

User-Defined Keywords

Biomimetic strategy
Biosensor
Covalent organic framework
Enzyme-nanozyme cascade
Nanozyme

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.cej.2023.146979

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@article{929a466eeaea42dc915ba03119c2bbf5,

title = "Bioinspired construction of histidine-doped porphyrin covalent organic framework nanozyme with enhanced peroxidase-like activity for sensitive uric acid detection",

abstract = "Imitating catalytic sites of natural enzymes is a promising approach to accurately improve the catalytic performances of nanozymes. Herein, inspired by the structure of natural horseradish peroxidase (HRP), three-component condensation strategy is unitized to design histidine (His) functionalized iron porphyrin covalent organic frameworks (Fe-COF-H3) nanozyme with peroxidase-like (POD-like) activity. The introduced His could form favorable microenvironment around the iron porphyrin structure, enhancing the catalytic capacity of COF nanozyme. Taking advantage of its excellent POD-like activity, Fe-COF-H3 was utilized as a carrier to immobilize urate oxidase (UOx) for the construction of enzyme-nanozyme cascade system (UOx@Fe-COF-H3). The UOx@Fe-COF-H3 was successfully applied for the colorimetric detection of uric acid (UA) in biological samples. This work not only offers a new insight to develop high-efficiency COF nanozymes, but also expands the application of COF nanozyme for the construction of enzyme-nanozyme cascade system.",

keywords = "Biomimetic strategy, Biosensor, Covalent organic framework, Enzyme-nanozyme cascade, Nanozyme",

author = "Chao Zhong and Cong Hu and Dan Ouyang and Akang Dan and Yanhui Zhong and Zongwei Cai and Zian Lin",

note = "This work was supported by the National Natural Science Foundation of China (22274021, 21974021, and 22036001). Publisher Copyright: {\textcopyright} 2023 Elsevier B.V.",

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T1 - Bioinspired construction of histidine-doped porphyrin covalent organic framework nanozyme with enhanced peroxidase-like activity for sensitive uric acid detection

AU - Zhong, Chao

AU - Hu, Cong

AU - Ouyang, Dan

AU - Dan, Akang

AU - Zhong, Yanhui

AU - Cai, Zongwei

AU - Lin, Zian

PY - 2023/12/1

Y1 - 2023/12/1

N2 - Imitating catalytic sites of natural enzymes is a promising approach to accurately improve the catalytic performances of nanozymes. Herein, inspired by the structure of natural horseradish peroxidase (HRP), three-component condensation strategy is unitized to design histidine (His) functionalized iron porphyrin covalent organic frameworks (Fe-COF-H3) nanozyme with peroxidase-like (POD-like) activity. The introduced His could form favorable microenvironment around the iron porphyrin structure, enhancing the catalytic capacity of COF nanozyme. Taking advantage of its excellent POD-like activity, Fe-COF-H3 was utilized as a carrier to immobilize urate oxidase (UOx) for the construction of enzyme-nanozyme cascade system (UOx@Fe-COF-H3). The UOx@Fe-COF-H3 was successfully applied for the colorimetric detection of uric acid (UA) in biological samples. This work not only offers a new insight to develop high-efficiency COF nanozymes, but also expands the application of COF nanozyme for the construction of enzyme-nanozyme cascade system.

AB - Imitating catalytic sites of natural enzymes is a promising approach to accurately improve the catalytic performances of nanozymes. Herein, inspired by the structure of natural horseradish peroxidase (HRP), three-component condensation strategy is unitized to design histidine (His) functionalized iron porphyrin covalent organic frameworks (Fe-COF-H3) nanozyme with peroxidase-like (POD-like) activity. The introduced His could form favorable microenvironment around the iron porphyrin structure, enhancing the catalytic capacity of COF nanozyme. Taking advantage of its excellent POD-like activity, Fe-COF-H3 was utilized as a carrier to immobilize urate oxidase (UOx) for the construction of enzyme-nanozyme cascade system (UOx@Fe-COF-H3). The UOx@Fe-COF-H3 was successfully applied for the colorimetric detection of uric acid (UA) in biological samples. This work not only offers a new insight to develop high-efficiency COF nanozymes, but also expands the application of COF nanozyme for the construction of enzyme-nanozyme cascade system.

KW - Biomimetic strategy

KW - Biosensor

KW - Covalent organic framework

KW - Enzyme-nanozyme cascade

KW - Nanozyme

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DO - 10.1016/j.cej.2023.146979

M3 - Journal article

AN - SCOPUS:85176231842

SN - 1385-8947

VL - 477

JO - Chemical Engineering Journal

JF - Chemical Engineering Journal

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Bioinspired construction of histidine-doped porphyrin covalent organic framework nanozyme with enhanced peroxidase-like activity for sensitive uric acid detection

Abstract

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