Bioinspired construction of histidine-doped porphyrin covalent organic framework nanozyme with enhanced peroxidase-like activity for sensitive uric acid detection

Chao Zhong, Cong Hu, Dan Ouyang, Akang Dan, Yanhui Zhong*, Zongwei Cai, Zian Lin*

*Corresponding author for this work

Research output: Contribution to journalJournal articlepeer-review

Abstract

Imitating catalytic sites of natural enzymes is a promising approach to accurately improve the catalytic performances of nanozymes. Herein, inspired by the structure of natural horseradish peroxidase (HRP), three-component condensation strategy is unitized to design histidine (His) functionalized iron porphyrin covalent organic frameworks (Fe-COF-H3) nanozyme with peroxidase-like (POD-like) activity. The introduced His could form favorable microenvironment around the iron porphyrin structure, enhancing the catalytic capacity of COF nanozyme. Taking advantage of its excellent POD-like activity, Fe-COF-H3 was utilized as a carrier to immobilize urate oxidase (UOx) for the construction of enzyme-nanozyme cascade system (UOx@Fe-COF-H3). The UOx@Fe-COF-H3 was successfully applied for the colorimetric detection of uric acid (UA) in biological samples. This work not only offers a new insight to develop high-efficiency COF nanozymes, but also expands the application of COF nanozyme for the construction of enzyme-nanozyme cascade system.

Original languageEnglish
Article number146979
JournalChemical Engineering Journal
Volume477
Early online date28 Oct 2023
DOIs
Publication statusPublished - 1 Dec 2023

Scopus Subject Areas

  • Chemistry(all)
  • Environmental Chemistry
  • Chemical Engineering(all)
  • Industrial and Manufacturing Engineering

User-Defined Keywords

  • Biomimetic strategy
  • Biosensor
  • Covalent organic framework
  • Enzyme-nanozyme cascade
  • Nanozyme

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