TY - JOUR
T1 - Bioinspired construction of histidine-doped porphyrin covalent organic framework nanozyme with enhanced peroxidase-like activity for sensitive uric acid detection
AU - Zhong, Chao
AU - Hu, Cong
AU - Ouyang, Dan
AU - Dan, Akang
AU - Zhong, Yanhui
AU - Cai, Zongwei
AU - Lin, Zian
N1 - This work was supported by the National Natural Science Foundation of China (22274021, 21974021, and 22036001).
Publisher Copyright:
© 2023 Elsevier B.V.
PY - 2023/12/1
Y1 - 2023/12/1
N2 - Imitating catalytic sites of natural enzymes is a promising approach to accurately improve the catalytic performances of nanozymes. Herein, inspired by the structure of natural horseradish peroxidase (HRP), three-component condensation strategy is unitized to design histidine (His) functionalized iron porphyrin covalent organic frameworks (Fe-COF-H3) nanozyme with peroxidase-like (POD-like) activity. The introduced His could form favorable microenvironment around the iron porphyrin structure, enhancing the catalytic capacity of COF nanozyme. Taking advantage of its excellent POD-like activity, Fe-COF-H3 was utilized as a carrier to immobilize urate oxidase (UOx) for the construction of enzyme-nanozyme cascade system (UOx@Fe-COF-H3). The UOx@Fe-COF-H3 was successfully applied for the colorimetric detection of uric acid (UA) in biological samples. This work not only offers a new insight to develop high-efficiency COF nanozymes, but also expands the application of COF nanozyme for the construction of enzyme-nanozyme cascade system.
AB - Imitating catalytic sites of natural enzymes is a promising approach to accurately improve the catalytic performances of nanozymes. Herein, inspired by the structure of natural horseradish peroxidase (HRP), three-component condensation strategy is unitized to design histidine (His) functionalized iron porphyrin covalent organic frameworks (Fe-COF-H3) nanozyme with peroxidase-like (POD-like) activity. The introduced His could form favorable microenvironment around the iron porphyrin structure, enhancing the catalytic capacity of COF nanozyme. Taking advantage of its excellent POD-like activity, Fe-COF-H3 was utilized as a carrier to immobilize urate oxidase (UOx) for the construction of enzyme-nanozyme cascade system (UOx@Fe-COF-H3). The UOx@Fe-COF-H3 was successfully applied for the colorimetric detection of uric acid (UA) in biological samples. This work not only offers a new insight to develop high-efficiency COF nanozymes, but also expands the application of COF nanozyme for the construction of enzyme-nanozyme cascade system.
KW - Biomimetic strategy
KW - Biosensor
KW - Covalent organic framework
KW - Enzyme-nanozyme cascade
KW - Nanozyme
UR - http://www.scopus.com/inward/record.url?scp=85176231842&partnerID=8YFLogxK
U2 - 10.1016/j.cej.2023.146979
DO - 10.1016/j.cej.2023.146979
M3 - Journal article
AN - SCOPUS:85176231842
SN - 1385-8947
VL - 477
JO - Chemical Engineering Journal
JF - Chemical Engineering Journal
M1 - 146979
ER -
