TY - JOUR
T1 - Biodegradation and removal of 3,4-dichloroaniline by Chlorella pyrenoidosa based on liquid chromatography-electrospray ionization-mass spectrometry
AU - Wang, Shujuan
AU - Poon, Karen
AU - CAI, Zongwei
N1 - Funding Information:
Acknowledgments The work was partly supported by research grant from the Beijing Normal University–Hong Kong Baptist University United International College. Financial support from the Faculty Research Fund of Hong Kong Baptist University (FRG2/10-11/029) is acknowledged.
PY - 2013/1
Y1 - 2013/1
N2 - 3,4-Dichloroaniline (3,4-DCA), widely used in the synthesis of dyes, textile and herbicides, is toxic to living organisms. The purpose of this study was to investigate the capability of green algae in degrading and removing 3,4-DCA in water. An environmentally ubiquitous green alga Chlorella pyrenoidosa was isolated from fresh aquatic environment. Then unicellular alga was incubated with 3,4-DCA at a concentration of 4. 6 μg/ mL in water. The residual concentration of 3,4-DCA in the medium and the metabolites were analyzed. A removal percentage of 78. 4 % was obtained over a 7-day period. Two major metabolites with less toxicity were identified as 3,4-dichloroformanilide and 3,4-dichloroacetanilide from the liquid chromatography-electrospray ionization-mass spectrometry analysis. The application of microalga C. pyrenoidosa may have potential for removing the environmental pollutant in aquatic environment.
AB - 3,4-Dichloroaniline (3,4-DCA), widely used in the synthesis of dyes, textile and herbicides, is toxic to living organisms. The purpose of this study was to investigate the capability of green algae in degrading and removing 3,4-DCA in water. An environmentally ubiquitous green alga Chlorella pyrenoidosa was isolated from fresh aquatic environment. Then unicellular alga was incubated with 3,4-DCA at a concentration of 4. 6 μg/ mL in water. The residual concentration of 3,4-DCA in the medium and the metabolites were analyzed. A removal percentage of 78. 4 % was obtained over a 7-day period. Two major metabolites with less toxicity were identified as 3,4-dichloroformanilide and 3,4-dichloroacetanilide from the liquid chromatography-electrospray ionization-mass spectrometry analysis. The application of microalga C. pyrenoidosa may have potential for removing the environmental pollutant in aquatic environment.
KW - Chlorella pyrenoidosa
KW - Green alga
KW - Isolation
KW - Metabolites
KW - Removal
UR - http://www.scopus.com/inward/record.url?scp=84871985419&partnerID=8YFLogxK
U2 - 10.1007/s11356-012-0995-9
DO - 10.1007/s11356-012-0995-9
M3 - Journal article
C2 - 22669566
AN - SCOPUS:84871985419
SN - 0944-1344
VL - 20
SP - 552
EP - 557
JO - Environmental Science and Pollution Research
JF - Environmental Science and Pollution Research
IS - 1
ER -