Biocompatible Porphyrin-Peptide Conjugates as Theranostic Agents Targeting the Epstein-Barr Virus

Xiong Li; Chen Xie; Shuai Zha; Wing Sze Tam; Mingjun Jiang; Ka Leung Wong

doi:10.1002/cplu.202200184

Biocompatible Porphyrin-Peptide Conjugates as Theranostic Agents Targeting the Epstein-Barr Virus

Xiong Li, Chen Xie, Shuai Zha, Wing Sze Tam, Mingjun Jiang, Ka Leung Wong^*

^*Corresponding author for this work

Department of Chemistry

Research output: Contribution to journal › Journal article › peer-review

2 Citations (Scopus)

Abstract

Epstein-Barr virus (EBV) is a common human-infected virus related to many diseases and cancers. Recently, some peptides have been found to serve targeting and therapeutic roles by inhibiting EBNA1, an oncoprotein of the EBV. We herein report the conjugation of the EBNA1-targeting peptides and porphyrins which can bring synergistic effects by both introducing more specific treatments (photodynamic therapy) and improving the biocompatibility of the photosensitizer and the peptides. One of our compounds exhibited significant photo-cytotoxicity where the Lethal Concentration 50 (LC₅₀)=6.1 μM in EBV-positive cells. Besides, in vitro cell imaging and co-staining can also be achieved simultaneously and suggested the binding inside nucleus.

Original language	English
Article number	e202200184
Journal	ChemPlusChem
Volume	87
Issue number	11
DOIs	https://doi.org/10.1002/cplu.202200184
Publication status	Published - Nov 2022

Scopus Subject Areas

Chemistry(all)

User-Defined Keywords

cell imaging
Epstein-Barr virus
photodynamic therapy
porphyrin
singlet oxygen

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1002/cplu.202200184

Cite this

@article{c428837dbdfd4afba447525cd4fd2ca3,

title = "Biocompatible Porphyrin-Peptide Conjugates as Theranostic Agents Targeting the Epstein-Barr Virus",

abstract = "Epstein-Barr virus (EBV) is a common human-infected virus related to many diseases and cancers. Recently, some peptides have been found to serve targeting and therapeutic roles by inhibiting EBNA1, an oncoprotein of the EBV. We herein report the conjugation of the EBNA1-targeting peptides and porphyrins which can bring synergistic effects by both introducing more specific treatments (photodynamic therapy) and improving the biocompatibility of the photosensitizer and the peptides. One of our compounds exhibited significant photo-cytotoxicity where the Lethal Concentration 50 (LC50)=6.1 μM in EBV-positive cells. Besides, in vitro cell imaging and co-staining can also be achieved simultaneously and suggested the binding inside nucleus.",

keywords = "cell imaging, Epstein-Barr virus, photodynamic therapy, porphyrin, singlet oxygen",

author = "Xiong Li and Chen Xie and Shuai Zha and Tam, {Wing Sze} and Mingjun Jiang and Wong, {Ka Leung}",

note = "Financial assistance from the Hong Kong Research Grants Council Grant No. 1230019, Dr. Mok Man Hung Endowed Professorship in Chemistry, HKBU – Durham Lanthanide Joint Research Center – Lanthanide, and from Health and Medical Research Fund 2019/2020 (18170022). Tools for Systems Medicine (SDF19-1011-P02) and the Centre for Medical Engineering of Molecular and Biological Probes (AoE/M-401/20) are gratefully acknowledged (K.-L.W.). Publisher Copyright: {\textcopyright} 2022 Wiley-VCH GmbH.",

year = "2022",

month = nov,

doi = "10.1002/cplu.202200184",

language = "English",

volume = "87",

journal = "ChemPlusChem",

issn = "2192-6506",

publisher = "Wiley-VCH Verlag",

number = "11",

}

TY - JOUR

T1 - Biocompatible Porphyrin-Peptide Conjugates as Theranostic Agents Targeting the Epstein-Barr Virus

AU - Li, Xiong

AU - Xie, Chen

AU - Zha, Shuai

AU - Tam, Wing Sze

AU - Jiang, Mingjun

AU - Wong, Ka Leung

N1 - Financial assistance from the Hong Kong Research Grants Council Grant No. 1230019, Dr. Mok Man Hung Endowed Professorship in Chemistry, HKBU – Durham Lanthanide Joint Research Center – Lanthanide, and from Health and Medical Research Fund 2019/2020 (18170022). Tools for Systems Medicine (SDF19-1011-P02) and the Centre for Medical Engineering of Molecular and Biological Probes (AoE/M-401/20) are gratefully acknowledged (K.-L.W.). Publisher Copyright: © 2022 Wiley-VCH GmbH.

PY - 2022/11

Y1 - 2022/11

N2 - Epstein-Barr virus (EBV) is a common human-infected virus related to many diseases and cancers. Recently, some peptides have been found to serve targeting and therapeutic roles by inhibiting EBNA1, an oncoprotein of the EBV. We herein report the conjugation of the EBNA1-targeting peptides and porphyrins which can bring synergistic effects by both introducing more specific treatments (photodynamic therapy) and improving the biocompatibility of the photosensitizer and the peptides. One of our compounds exhibited significant photo-cytotoxicity where the Lethal Concentration 50 (LC50)=6.1 μM in EBV-positive cells. Besides, in vitro cell imaging and co-staining can also be achieved simultaneously and suggested the binding inside nucleus.

AB - Epstein-Barr virus (EBV) is a common human-infected virus related to many diseases and cancers. Recently, some peptides have been found to serve targeting and therapeutic roles by inhibiting EBNA1, an oncoprotein of the EBV. We herein report the conjugation of the EBNA1-targeting peptides and porphyrins which can bring synergistic effects by both introducing more specific treatments (photodynamic therapy) and improving the biocompatibility of the photosensitizer and the peptides. One of our compounds exhibited significant photo-cytotoxicity where the Lethal Concentration 50 (LC50)=6.1 μM in EBV-positive cells. Besides, in vitro cell imaging and co-staining can also be achieved simultaneously and suggested the binding inside nucleus.

KW - cell imaging

KW - Epstein-Barr virus

KW - photodynamic therapy

KW - porphyrin

KW - singlet oxygen

UR - http://www.scopus.com/inward/record.url?scp=85136898955&partnerID=8YFLogxK

U2 - 10.1002/cplu.202200184

DO - 10.1002/cplu.202200184

M3 - Journal article

C2 - 36036545

AN - SCOPUS:85136898955

SN - 2192-6506

VL - 87

JO - ChemPlusChem

JF - ChemPlusChem

IS - 11

M1 - e202200184

ER -

Biocompatible Porphyrin-Peptide Conjugates as Theranostic Agents Targeting the Epstein-Barr Virus

Abstract

Scopus Subject Areas

User-Defined Keywords

UN SDGs

Access to Document

Other files and links

Cite this