TY - JOUR
T1 - Bile Acid Profiles Are Distinct among Patients with Different Etiologies of Chronic Liver Disease
AU - Sang, Chao
AU - Wang, Xiaoning
AU - Zhou, Kejun
AU - Sun, Tao
AU - Bian, Hua
AU - Gao, Xin
AU - Wang, Yixing
AU - Zhang, Hua
AU - Jia, Wei
AU - Liu, Ping
AU - Xie, Guoxiang
AU - Chen, Tianlu
N1 - Funding Information:
We wish to thank the research coordinators of the participating hospitals for their assistance in collecting clinical data and samples. This study was financially supported by the National Key R&D Program of China (2019YFA0802300 and 2017YFC0906800), the National Natural Science Foundation of China (31972935), and the Shenzhen Science, Technology and Innovation Commission (2020(82)).
Publisher Copyright:
© 2021 American Chemical Society
PY - 2021/5/7
Y1 - 2021/5/7
N2 - A significant increase of bile acid (BA) levels has been recognized as a general metabolic phenotype of diverse liver diseases. Monitoring of BA profiles has been proposed for etiology differentiation on liver injury. Here, we quantitatively profiled serum BAs of healthy controls and 719 patients with chronic liver disease of five etiologies, hepatitis B virus (HBV), hepatitis C virus (HCV), nonalcoholic steatohepatitis (NASH), alcohol-induced liver disease (ALD), and primary biliary cirrhosis (PBC), and investigated the generality and specificity of different etiologies. The raw data have been deposited into MetaboLights (ID: MTBLS2459). We found that patients with HBV, HCV, and NASH appeared to be more similar, and ALD and PBC patients clustered together. BA profiles, consisting of a total concentration of the 21 quantified BAs [total BAs (TBAs)], 21 BA proportions, and 24 BA relevant variables, were highly different among the etiologies. Specifically, the total BAs was higher in ALD and PBC patients compared with the other three groups. The proportion of conjugated deoxycholates was the highest in HBV-infected patients. The ratio of 12α-hydroxylated (12α-OH) to non-12α-OH BAs was the highest in NASH patients. The proportion of taurine-conjugated BAs was the highest in ALD patients. For PBC patients, the proportion of ursodeoxycholate species was the highest, and the ratio of primary to secondary BAs was the lowest. Comparatively, the difference of BA profiles among cirrhosis patients was consistent but weaker than that of all patients. The correlations between BA profiles and clinical indices were also quite different in different pathological groups, both in all patients and in patients with cirrhosis. Overall, our findings suggested that BA compositions are distinct among patients with different etiologies of chronic liver disease, and some BA-relevant variables are of clinical potentials for liver injury type differentiation, although further validations on more etiologies and populations are needed.
AB - A significant increase of bile acid (BA) levels has been recognized as a general metabolic phenotype of diverse liver diseases. Monitoring of BA profiles has been proposed for etiology differentiation on liver injury. Here, we quantitatively profiled serum BAs of healthy controls and 719 patients with chronic liver disease of five etiologies, hepatitis B virus (HBV), hepatitis C virus (HCV), nonalcoholic steatohepatitis (NASH), alcohol-induced liver disease (ALD), and primary biliary cirrhosis (PBC), and investigated the generality and specificity of different etiologies. The raw data have been deposited into MetaboLights (ID: MTBLS2459). We found that patients with HBV, HCV, and NASH appeared to be more similar, and ALD and PBC patients clustered together. BA profiles, consisting of a total concentration of the 21 quantified BAs [total BAs (TBAs)], 21 BA proportions, and 24 BA relevant variables, were highly different among the etiologies. Specifically, the total BAs was higher in ALD and PBC patients compared with the other three groups. The proportion of conjugated deoxycholates was the highest in HBV-infected patients. The ratio of 12α-hydroxylated (12α-OH) to non-12α-OH BAs was the highest in NASH patients. The proportion of taurine-conjugated BAs was the highest in ALD patients. For PBC patients, the proportion of ursodeoxycholate species was the highest, and the ratio of primary to secondary BAs was the lowest. Comparatively, the difference of BA profiles among cirrhosis patients was consistent but weaker than that of all patients. The correlations between BA profiles and clinical indices were also quite different in different pathological groups, both in all patients and in patients with cirrhosis. Overall, our findings suggested that BA compositions are distinct among patients with different etiologies of chronic liver disease, and some BA-relevant variables are of clinical potentials for liver injury type differentiation, although further validations on more etiologies and populations are needed.
KW - alcohol-induced liver disease
KW - Bile acids
KW - HBV
KW - HCV
KW - NASH
KW - primary biliary cirrhosis
UR - http://www.scopus.com/inward/record.url?scp=85104966605&partnerID=8YFLogxK
U2 - 10.1021/acs.jproteome.0c00852
DO - 10.1021/acs.jproteome.0c00852
M3 - Journal article
C2 - 33754726
AN - SCOPUS:85104966605
SN - 1535-3893
VL - 20
SP - 2340
EP - 2351
JO - Journal of Proteome Research
JF - Journal of Proteome Research
IS - 5
ER -
