TY - JOUR
T1 - Berberine ameliorates DSS-induced intestinal mucosal barrier dysfunction through microbiota-dependence and Wnt/β-catenin pathway
AU - Dong, Yalan
AU - Fan, Heng
AU - Zhang, Zhe
AU - Jiang, Feng
AU - Li, Mingyue
AU - Zhou, Haifeng
AU - Guo, Weina
AU - Zhang, Zili
AU - Kang, Zhenyu
AU - Gui, Yang
AU - Shou, Zhexing
AU - Li, Junyi
AU - Zhu, Rui
AU - Fu, Yu
AU - Sarapultsev, Alexey
AU - Wang, Huafang
AU - Luo, Shanshan
AU - Zhang, Ge
AU - Hu, Desheng
N1 - Funding Information:
This work was financially supported by grants from the National Key Research and Development Program of China (Project No. 2019YFC1316204), the National Natural Science Foundation of China (Nos. 31770983, 81974249, 81601747, and 82070572), and the Natural Science Foundation of Hubei Province (No. 2020BHB016).
Publisher Copyright:
© The author(s). See http://ivysp.
PY - 2022/1/16
Y1 - 2022/1/16
N2 - Ulcerative colitis (UC) is an idiopathic, chronic inflammatory disorder of the colon, and it has become one of the world-recognized medical problems as it is recurrent and refractory. Berberine (BBR) is an effective drug for UC treatment. However, the underlying mechanism and targets remain obscure. In this study, we systematically investigated the therapeutic effect and its mechanism of BBR in ameliorating DSS-induced mouse colitis. Expectedly, the colon inflammation was significantly relieved by BBR, and microbiota depletion by antibiotic cocktail significantly reversed the therapeutic effect. Further studies showed that BBR can regulate the abundance and component of bacteria, reestablish the broken chemical and epithelial barriers. Meanwhile, BBR administration dramatically decreased ILC1 and Th17 cells, and increased Tregs as well as ILC3 in colonic tissue of DSS-induced mice, and it was able to regulate the expression of various immune factors at the mRNA level. Moreover, a proteomic study revealed that Wnt/β-catenin pathway was remarkably enhanced in colonic tissue of BBR-treated mice, and the therapeutic effect of BBR was disappeared after the intervention of Wnt pathway inhibitor FH535. These results substantially revealed that BBR restores DSS-induced colon inflammation in a microbiota-dependent manner, and BBR performs its protective roles in colon by maintaining the structure and function of the intestinal mucosal barrier, regulating the intestinal mucosal immune homeostasis and it works through the Wnt/β-catenin pathway. Importantly, these findings also provided the proof that BBR serves as a potential gut microbiota modulator and mucosal barrier protector for UC prevention and therapy.
AB - Ulcerative colitis (UC) is an idiopathic, chronic inflammatory disorder of the colon, and it has become one of the world-recognized medical problems as it is recurrent and refractory. Berberine (BBR) is an effective drug for UC treatment. However, the underlying mechanism and targets remain obscure. In this study, we systematically investigated the therapeutic effect and its mechanism of BBR in ameliorating DSS-induced mouse colitis. Expectedly, the colon inflammation was significantly relieved by BBR, and microbiota depletion by antibiotic cocktail significantly reversed the therapeutic effect. Further studies showed that BBR can regulate the abundance and component of bacteria, reestablish the broken chemical and epithelial barriers. Meanwhile, BBR administration dramatically decreased ILC1 and Th17 cells, and increased Tregs as well as ILC3 in colonic tissue of DSS-induced mice, and it was able to regulate the expression of various immune factors at the mRNA level. Moreover, a proteomic study revealed that Wnt/β-catenin pathway was remarkably enhanced in colonic tissue of BBR-treated mice, and the therapeutic effect of BBR was disappeared after the intervention of Wnt pathway inhibitor FH535. These results substantially revealed that BBR restores DSS-induced colon inflammation in a microbiota-dependent manner, and BBR performs its protective roles in colon by maintaining the structure and function of the intestinal mucosal barrier, regulating the intestinal mucosal immune homeostasis and it works through the Wnt/β-catenin pathway. Importantly, these findings also provided the proof that BBR serves as a potential gut microbiota modulator and mucosal barrier protector for UC prevention and therapy.
KW - Berberine
KW - Intestinal mucosal barrier
KW - Microbiota
KW - Ulcerative colitis
KW - Wnt/β-catenin pathway
UR - http://www.scopus.com/inward/record.url?scp=85126400581&partnerID=8YFLogxK
U2 - 10.7150/ijbs.65476
DO - 10.7150/ijbs.65476
M3 - Journal article
C2 - 35280677
SN - 1449-2288
VL - 18
SP - 1381
EP - 1397
JO - International Journal of Biological Sciences
JF - International Journal of Biological Sciences
IS - 4
ER -