Baicalein antagonizes rotenone-induced apoptosis in dopaminergic SH-SY5Y cells related to Parkinsonism

Ju Xian Song, Mandy Yuen Man Choi, Kavin Chun Kit Wong, Winkie Wing Yan Chung, Stephen Cho Wing Sze, Tzi Bun Ng, Kalin Yan Bo Zhang*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Background
Two active compounds, baicalein and its glycoside baicalin were found in the dried root of Scutellaria baicalensis Georgi, and reported to be neuroprotective in vitro and in vivo. This study aims to evaluate the protective effects of baicalein on the rotenone-induced apoptosis in dopaminergic SH-SY5Y cells related to parkinsonism.

Methods
Cell viability and cytotoxicity were determined by MTT assay. The degree of nuclear apoptosis was evaluated with a fluorescent DNA-binding probe Hoechst 33258. The production of reactive oxidative species (ROS) and loss of mitochondrial membrane potential (ΔΨm) were determined by fluorescent staining with DCFH-DA and Rhodanmine 123, respectively. The expression of Bax, Bcl-2, cleaved caspase-3 and phosphorylated ERK1/2 was determined by the Western blots.

Results
Baicalein significantly increased viability and decreased rotenone-induced death of SH-SY5Y cells in a dose-dependent manner. Pre- and subsequent co-treatment with baicalein preserved the cell morphology and attenuated the nuclear apoptotic characteristics triggered by rotenone. Baicalein antagonized rotenone-induced overproduction of ROS, loss of ΔΨm, the increased expression of Bax, cleaved caspase-3 and phosphorylated ERK1/2 and the decreased expression of Bcl-2.

Conclusion
The antioxidative effect, mitochondrial protection and modulation of anti-and pro-apoptotic proteins are related to the neuroprotective effects of baicalein against rotenone induced cell death in SH-SY5Y cells.
Original languageEnglish
Article number1
JournalChinese Medicine
Volume7
DOIs
Publication statusPublished - 21 Jan 2012
Externally publishedYes

Scopus Subject Areas

  • Medicine(all)

User-Defined Keywords

  • Reactive Oxygen Species
  • Rotenone Treatment
  • Scutellaria Baicalensis
  • Nuclear Apoptosis
  • Baicalein Treatment

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